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AB193717

Mouse beta IGH3 ELISA Kit

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Mouse beta IGH3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse beta IGH3 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Tgfbi

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Sandwich ELISA - Mouse beta IGH3 ELISA Kit (AB193717)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse beta IGH3 ELISA Kit (AB193717)

Typical Standard Curve using ab193717 BIGH3 Mouse ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 3 pg/mL

Range

3 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's beta IGH3 mouse ELISA Kit (ab193717) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse BIGH3 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse beta IGH3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures beta IGH3 present in the samples. The wells are washed and biotinylated anti-mouse beta IGH3 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of beta IGH3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get better reproducibility in only 90 minutes with Mouse beta IG H3 ELISA Kit (ab206987) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "132 - 148 %", "average": "= 140.1" }, { "sample": "Plasma", "range": "115 - 150 %", "average": "= 136" }, { "sample": "Cell culture media", "range": "137 - 149 %", "average": "= 143.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193717-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193717-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193717-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB193717-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193717-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB193717-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193717-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse beta-IGH3 Microplate (12 strips x 8 wells)", "number":"AB193717-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse beta-IGH3 standards (lyophilized)", "number":"AB193717-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse beta-IGH3 detection antibody", "number":"AB193717-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in cell adhesion (PubMed : 8024701). May play a role in cell-collagen interactions (By similarity).
See full target information Tgfbi
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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websiteProtocolBooklet
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Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB206987

Mouse beta IG H3 ELISA Kit

elisa-kits

mouse-beta-ig-h3-elisa-kit-ab206987

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