JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277398

Mouse Beta nerve growth factor ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Beta nerve growth factor ELISA Kit is a Sandwich ELISA for the measurement of Mouse Beta nerve growth factor in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Ngfb, Ngf, Beta-nerve growth factor, Beta-NGF

1 Images
Sandwich ELISA - Mouse Beta nerve growth factor ELISA Kit (AB277398)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Beta nerve growth factor ELISA Kit (AB277398)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 40 pg/mL

Range

0.041 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB212166

Human BDNF ELISA Kit

SimpleStep

ELISA - Human BDNF ELISA Kit (AB212166)

  • 4
  • 2
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

  • 5
  • 35
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse Beta nerve growth factor ELISA Kit (ab277398) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Beta nerve growth factor in serum (mouse beta-NGF concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse Beta nerve growth factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse Beta nerve growth factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse Beta nerve growth factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse Beta nerve growth factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "81 - 149 %", "average": "= 117.8" }, { "sample": "Plasma", "range": "78 - 148 %", "average": "= 108.8" }, { "sample": "Cell culture media", "range": "79 - 139 %", "average": "= 108.4" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277398-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277398-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277398-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse Beta nerve growth factor coated Microplate", "number":"AB277398-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB277398-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277398-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse Beta nerve growth factor Antibody", "number":"AB277398-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277398-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Beta nerve growth factor standard protein (Lyophilized)", "number":"AB277398-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277398-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta nerve growth factor (beta-NGF) is a significant protein that functions in the development and maintenance of the nervous system. It is also known as NGF or NGFB and has a molecular mass of around 26 kDa. This protein is highly expressed in a wide range of tissues including the brain skin and various glands. Researchers often use assays like growth factor assays and NGF ELISA kits to measure beta-NGF levels given its importance in several physiological processes.
Biological function summary

Beta nerve growth factor plays an important role in the growth survival and differentiation of neurons. As a member of the neurotrophin family it binds to receptors like TrkA and p75NTR on the surface of nerve cells. This binding activity helps to promote neuronal health and function especially in the peripheral and central nervous systems. While beta-NGF traditionally acts independently it also interacts with other signaling molecules to trigger cascades that support cell survival and neural connections.

Pathways

Beta-NGF prominently participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the cell surface to the DNA in the nucleus therefore regulating gene expression linked to cell growth and survival. In these contexts beta-NGF works closely with proteins like Akt and ERK enhancing neuron resilience and promoting restorative mechanisms across the nervous system.

Beta nerve growth factor has been connected to neurodegenerative diseases like Alzheimer's disease and chronic pain conditions. Alterations in beta-NGF expression or signaling can impact neuronal survival contributing to the progression of these disorders. In Alzheimer's disease for example changes in NGF interactions with proteins like amyloid-beta and tau may exacerbate neurodegeneration. Similarly in pain syndromes disruptions in beta-NGF signaling can influence pain perception through pathways involving other neurotrophic factors.
style
left-column

Product protocols

style
left-column

Target data

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 20036257). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (PubMed : 22649032). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse (PubMed : 22155786). In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (PubMed : 20036257). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (By similarity). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer (PubMed : 22649032, PubMed : 26144237). The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (PubMed : 22649032).
See full target information Ngf
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced healthcare materials 12:e2202127 PubMed36325948

2022

Lipid Nanoparticle Delivery of Chemically Modified NGF mRNA Alleviates Peripheral Neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Yu,Zheng Yang,Yu Zhang,Jia Xia,Jiahui Zhang,Qi Han,Hang Yu,Chengbiao Wu,Yingjie Xu,Wei Xu,Wen Yang
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com