AB255722

Mouse Betacellulin ELISA Kit (BTC)

Recombinant
SimpleStep
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Mouse Betacellulin ELISA Kit (BTC) is a single-wash 90-min Simplestep used to quantify Mouse Betacellulin (BTC) with a sensitivity of 1.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Bcn, Btc, Probetacellulin

3 Images

Sandwich ELISA - Mouse Betacellulin ELISA Kit (BTC) (AB255722)

sELISA

Supplier Data

Sandwich ELISA - Mouse Betacellulin ELISA Kit (BTC) (AB255722)

Example of mouse Betacellulin standard curve in Sample Diluent NS.

The Betacellulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Betacellulin ELISA Kit (BTC) (AB255722)

Interpolated concentrations of native Betacellulin in mouse kidney tissue culture supernatant samples.

The concentrations of Betacellulin were measured in duplicates, interpolated from the Betacellulin standard curves and corrected for sample dilution. Undiluted samples are as follows : kidney supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Betacellulin concentration was determined to be 349 pg/mL in kidney tissue culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Betacellulin ELISA Kit (BTC) (AB255722)

Interpolated concentrations of spike Betacellulin in mouse serum, and plasma samples.

The concentrations of Betacellulin were measured in duplicates, interpolated from the Betacellulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.6 pg/mL

Range

4.69 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Betacellulin ELISA Kit (BTC) (ab255722) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Betacellulin (BTC) protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Betacellulin (BTC) with 1.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Betacellulin (BTC) is an EGF growth factor family member that binds to EGF Receptor family members. BTC is expressed as a 177 amino acid precursor form that is a single-pass transmembrane protein (this form is called pro-betacellulin). Subsequent cleavage produces the soluble and secreted 79 amino acid betacellulin fragment from the extracellular portion of pro-betacellulin. This assay reacts with the soluble betacellulin protein, reactivity with pro-betacellulin was not determined.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 103 %", "average": "= 102" }, { "sample": "Serum", "range": "116 - 125 %", "average": "= 121" }, { "sample": "EDTA Plasma", "range": "112 - 119 %", "average": "= 116" }, { "sample": "Cell culture media", "range": "107 - 114 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "72 - 81 %", "average": "= 76" }, { "sample": "Citrate plasma", "range": "105 - 112 %", "average": "= 108" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The BTC protein also known as betacellulin is a member of the epidermal growth factor (EGF) family that plays an important role in cell signaling. It is a growth factor with a molecular weight of approximately 32 kDa. BTC is expressed in a range of tissues including the pancreas kidney and small intestine. This protein binds to and activates the EGF receptor (EGFR) stimulating various cellular processes. Researchers often use the BTC mouse and BTC chimeras to study its function and physiological roles due to their relevance to human biology.

Biological function summary

BTC plays an important role in regulating cell proliferation especially in the pancreas where it influences beta-cell growth and regeneration. It operates as part of a signaling complex that interacts with other members of the EGF family impacting various cellular responses. Researchers employ experimental tools like BTC ELISA kits to quantify BTC levels in biological samples aiding in the exploration of its biological functions and mechanisms. BTC’s ability to modulate growth pathways makes it a significant molecule under investigation.

Pathways

BTC participates in the EGF/EGFR signaling pathway which is essential for controlling cellular growth and differentiation. This pathway involves the interaction of BTC with other ligands such as EGF and transforming growth factor-alpha (TGF-α) altering downstream signaling events. BTC further contributes to the activation of the MAPK/ERK pathway a critical process for transmitting signals from the cell surface to the DNA in the nucleus. Through these pathways BTC impacts cellular activities and balances multiple biological functions.

BTC is linked to conditions such as diabetes and various cancers. In diabetes BTC is associated with pancreatic function particularly influencing insulin production through its role in beta-cell regulation. In cancer abnormal BTC expression or its interactions with EGFR signaling components can lead to uncontrolled cell proliferation. Researchers study BTC’s interactions with proteins like EGF and TGF-α to understand its involvement in these disorders as therapeutic targeting of this axis holds potential for treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

See full target information Btc

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com