Mouse Biglycan ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Biglycan with a sensitivity of 193 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 546.88 - 35000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 193 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
Biglycan, Bone/cartilage proteoglycan I, PG-S1, Bgn
What's included?
Recommended products
Mouse Biglycan ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Biglycan with a sensitivity of 193 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 546.88 - 35000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 193 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 3.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 5.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 88 | Range 80 - 95 % |
Sample type EDTA Plasma | Average % = 103 | Range 85 - 118 % |
Sample type Tissue Extracts | Average % = 91 | Range 83 - 116 % |
Sample type Citrate plasma | Average % = 97 | Range 84 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Biglycan ELISA kit (ab275906) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Biglycan protein in mouse serum, plasma and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Biglycan with 193 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Biglycan is a proteoglycan found in the extracellular matrix and plays a key role in the maintenance of bone, cartilage, and tendons. Along with its structural role, biglycan plays a crucial role in inflammatory signaling during stress and tissue injury. It is currently under investigation as a potential target for clinical treatment of muscular dystrophies and certain inflammatory diseases such as osteoarthritis. The antibodies for this kit were raised against a recombinant protein representing the majority of the c-terminal portion of the mature protein. This protein is homologous throughout Mammalia, so cross-reactivities are likely for samples from many species.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Biglycan also known as BGN is a small leucine-rich proteoglycan with a molecular weight of roughly 38 kDa. Researchers frequently observe Biglycan in tissues such as bone cartilage and vasculature. The protein interacts with several other molecules in the extracellular matrix (ECM) including collagen and elastin to maintain the structural integrity of tissues. Through these interactions Biglycan plays an important role in supporting cell attachment and matrix organization.
- Biological function summary
The protein Biglycan influences cellular processes such as growth and differentiation. It is part of a complex network within the ECM that modulates cell signaling. Biglycan interacts with growth factors like transforming growth factor-beta (TGF-beta) and binds to Toll-like receptors (TLRs) affecting immune responses. These capabilities reveal Biglycan’s involvement in tissue development and repair.
- Pathways
Extracellular matrix modulation shows a significant role for Biglycan. It is involved in the TGF-beta signaling pathway and the inflammatory pathway mediated by TLRs. Through these pathways Biglycan interacts with proteins such as decorin and fibromodulin contributing to the regulation of collagen fibrillogenesis and inflammatory responses. Its role in these pathways highlights Biglycan’s influence in cellular communication and matrix regulation.
- Associated diseases and disorders
Biglycan has associations with osteoarthritis and muscular dystrophy. In osteoarthritis researchers observe altered expression levels of Biglycan which affects cartilage integrity. In muscular dystrophy the protein’s connection to dystrophin an important structural protein is noted. These associations illustrate Biglycan's impact on disease progression and its potential as a therapeutic target.
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3 product images
Sandwich ELISA - Mouse Biglycan ELISA Kit (ab275906)
Example of mouse Biglycan standard curve in 1X Cell Extraction Buffer PTR.
The Biglycan standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Biglycan ELISA Kit (ab275906)
Example of mouse Biglycan standard curve in Sample Diluent NS.
The Biglycan standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Biglycan ELISA Kit (ab275906)
Interpolated concentrations of native Biglycan in mouse articular cartilage tissue extract based on a 75 μg/mL extract load.
The concentrations of Biglycan were measured in duplicate and interpolated from the Biglycan standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Biglycan concentration was determined to be 5,464.65 pg/mL.
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