AB119582

Mouse BMP2 ELISA Kit

Name: Mouse BMP2 ELISA Kit (ab119582) | Abcam
Brand: Abcam Limited
SKU: AB119582
Price: 780 USD
Availability: InStock

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(9 Publications)

Mouse BMP2 ELISA Kit is a Sandwich ELISA for the measurement of Mouse BMP2 in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Bmp-2, Bmp2, Bone morphogenetic protein 2, BMP-2, Bone morphogenetic protein 2A, BMP-2A

1 Images

Sandwich ELISA - Mouse BMP2 ELISA Kit (AB119582)

sELISA

Supplier Data

Sandwich ELISA - Mouse BMP2 ELISA Kit (AB119582)

Representative Standard Curve using ab119582.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

62.5 - 4000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's mouse BMP2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse BMP2 in bone tissue, cell culture supernatants and serum.

A BMP2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BMP2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse BMP2 amount of sample captured in plate.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119582-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119582-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse BMP2 antibody", "number":"AB119582-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse BMP2 antibody Microplate (12 x 8 wells)", "number":"AB119582-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119582-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119582-CMP09", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse BMP2 standard", "number":"AB119582-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB119582-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119582-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119582-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119582-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119582-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed : 31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation. Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (PubMed : 29148101).

See full target information Bmp2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 50:10526-10543 PubMed36134711

2022

LncRNA-Smad7 mediates cross-talk between Nodal/TGF-β and BMP signaling to regulate cell fate determination of pluripotent and multipotent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Kong,Kun Yan,Pujuan Deng,Haipeng Fu,Hongyao Sun,Wenze Huang,Shuangying Jiang,Junbiao Dai,Qiangfeng Cliff Zhang,Jun-Jie Gogo Liu,Qiaoran Xi

Bioactive materials 6:1318-1329 PubMed33210025

2020

A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Minqi Wang,Hanjun Li,Yiqi Yang,Kai Yuan,Feng Zhou,Haibei Liu,Qinghui Zhou,Shengbing Yang,Tingting Tang

International journal of nanomedicine 15:6563-6577 PubMed32982218

2020

Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Wu,Guanying Gao,Yan Xu

Aging 12:3617-3625 PubMed32096768

2020

Inflammatory macrophages facilitate mechanical stress-induced osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhang,Le Huan,Tao Xu,Guozheng Li,Bing Zheng,Hong Zhao,Yongfei Guo,Jiangang Shi,Jingchuan Sun,Aimin Chen

Experimental and therapeutic medicine 18:3659-3666 PubMed31602244

2019

MicroRNA-410 participates in the pathological process of postmenopausal osteoporosis by downregulating bone morphogenetic protein-2.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Wenbin Ding,Fang Ji,Dajiang Wu

PloS one 13:e0202857 PubMed30142209

2018

Bone marrow stromal cells from low-turnover osteoporotic mouse model are less sensitive to the osteogenic effects of fluvastatin.

Applications

Unspecified application

Species

Unspecified reactive species

Yukari Oda,Hodaka Sasaki,Tadashi Miura,Takuya Takanashi,Yoshitaka Furuya,Masao Yoshinari,Yasutomo Yajima

Experimental and therapeutic medicine 15:4298-4308 PubMed29849774

2018

Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhui Wang,Huimei Zang,Dongsheng Zhou

Biochemical and biophysical research communication 491:154-158 PubMed28711495

2017

Evidence for constitutive bone morphogenetic protein-2 secretion by M1 macrophages: Constitutive auto/paracrine osteogenic signaling by BMP-2 in M1 macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Prabhatchandra R Dube,Lutz Birnbaumer,Guillermo Vazquez

PloS one 8:e61600 PubMed23593489

2013

Single bout short duration fluid shear stress induces osteogenic differentiation of MC3T3-E1 cells via integrin β1 and BMP2 signaling cross-talk.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Mai,Zhuli Peng,Sihan Wu,Jinglan Zhang,Lin Chen,Huangyou Liang,Ding Bai,Guangmei Yan,Hong Ai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse BMP2 ELISA Kit

Sandwich ELISA - Mouse BMP2 ELISA Kit (AB119582)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (9)

Product promise