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AB267576

Mouse BMP9 ELISA Kit

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(3 Publications)

Mouse BMP9 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse BMP9 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Bmp9, Gdf2, Growth/differentiation factor 2, GDF-2, Bone morphogenetic protein 9, BMP-9

1 Images
Sandwich ELISA - Mouse BMP9 ELISA Kit (AB267576)
  • sELISA

Unknown

Sandwich ELISA - Mouse BMP9 ELISA Kit (AB267576)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 4 pg/mL

Range

4.92 - 1200 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse BMP9 ELISA Kit is designed for the quantitative determination of BMP9 in cell culture supernatants, plasma and serum samples.

∆ Note: Mouse BMP9 concentration is low in normal serum/plasma, and may not be detectable in this assay.

This assay employs an antibody specific for mouse BMP9 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse BMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit detects mouse and human BMP9. Other species not determined.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "75 - 89 %", "average": "= 80.73" }, { "sample": "Plasma", "range": "72 - 93 %", "average": "= 79.42" }, { "sample": "Cell culture media", "range": "74 - 102 %", "average": "= 83.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267576-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267576-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267576-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse BMP9 coated Microplate (12 x 8 wells)", "number":"AB267576-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267576-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267576-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse BMP9 Standard (Lyophilized)", "number":"AB267576-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267576-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267576-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse BMP9 Detection Antibody", "number":"AB267576-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Potent circulating inhibitor of angiogenesis (By similarity). Signals through the type I activin receptor ACVRL1 but not other Alks (PubMed : 25751889). Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG (PubMed : 23300529).
See full target information Gdf2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 238:2625-2637 PubMed37661654

2023

Effect of the secretome of mesenchymal stem cells overexpressing BMP-9 on osteoblast differentiation and bone repair.

Applications

Unspecified application

Species

Unspecified reactive species

Robson Diego Calixto,Gileade Pereira Freitas,Paola Gomes Souza,Jaqueline Isadora Reis Ramos,Isabela Cristine Santos,Fabiola Singaretti de Oliveira,Adriana Luisa Gonçalves Almeida,Adalberto Luiz Rosa,Marcio Mateus Beloti

Biology 12: PubMed37627031

2023

Effect of Mesenchymal Stem Cells Overexpressing BMP-9 Primed with Hypoxia on BMP Targets, Osteoblast Differentiation and Bone Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Emanuella Rocha Moura Paz,Leticia Faustino Adolpho,Jaqueline Isadora Reis Ramos,Rayana Longo Bighetti-Trevisan,Robson Diego Calixto,Fabiola Singaretti Oliveira,Adriana Luisa Gonçalves Almeida,Marcio Mateus Beloti,Adalberto Luiz Rosa

Gene therapy 28:748-759 PubMed33686254

2021

Mesenchymal stem cells overexpressing BMP-9 by CRISPR-Cas9 present high in vitro osteogenic potential and enhance in vivo bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Gileade P Freitas,Helena B Lopes,Alann T P Souza,Maria Paula O Gomes,Georgia K Quiles,Jonathan Gordon,Coralee Tye,Janet L Stein,Gary S Stein,Jane B Lian,Marcio M Beloti,Adalberto L Rosa
View all publications
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websiteProtocolBooklet
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