AB279405

Mouse Bone Sialoprotein ELISA Kit (IBSP)

Recombinant
SimpleStep
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Mouse Bone Sialoprotein ELISA Kit (IBSP) is a single-wash 90-min Simplestep used to quantify Mouse Bone Sialoprotein (IBSP) with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Integrin-binding sialoprotein, Bone sialoprotein 2, Bone sialoprotein II, Cell-binding sialoprotein, BSP II, Ibsp

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Sandwich ELISA - Mouse Bone Sialoprotein ELISA Kit (IBSP) (AB279405)

sELISA

Supplier Data

Sandwich ELISA - Mouse Bone Sialoprotein ELISA Kit (IBSP) (AB279405)

Interpolated concentrations of native Bone Sialoprotein in mouse serum and plasma samples.

The concentrations of Bone Sialoprotein were measured in duplicates, interpolated from the Bone Sialoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (heparin) 5% and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bone Sialoprotein concentration was determined to be 20.68 ng/mL in serum, 18.45 ng/mL in plasma (citrate) and 32.88 ng/mL in plasma (heparin), and 21.71 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse Bone Sialoprotein ELISA Kit (IBSP) (AB279405)

Example of mouse Bone Sialoprotein standard curve in Sample Diluent NS.

The Bone Sialoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6 pg/mL

Range

37.5 - 2400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Bone Sialoprotein ELISA kit (ab279405) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Bone Sialoprotein protein in mouse serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Bone Sialoprotein with 6 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Bone Sialoprotein, also known as IBSP, is a matrix protein that plays a role in mineralization of bone and other calcified structures. The exact function of this protein is currently under investigation. The immunogen with which the antibodies in this kit were raised is composed of the full-length mature protein. This protein is not homologous with other species; as such, it is not expected to cross-react.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]

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Recovery

[ { "sample": "Serum", "range": "85 - 109 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "85 - 112 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "106 - 120 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "104 - 115 %", "average": "= 111" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279405-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB279405-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB279405-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Bone Sialoprotein (IBSP) Capture Antibody", "number":"AB279405-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB279405-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Bone Sialoprotein (IBSP) Lyophilized Recombinant Protein", "number":"AB279405-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Bone Sialoprotein (IBSP) Detector Antibody", "number":"AB279405-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB279405-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB279405-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB279405-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB279405-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bone Sialoprotein (BSP) is a non-collagenous glycoprotein of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family with a mass around 70-80 kDa. Its expression mainly takes place in mineralized tissues like bone and dentin highlighting its importance in skeletal biology. BSP has a strong affinity for calcium and hydroxyapatite allowing it to play binding and structural roles in bone matrix formation.

Biological function summary

The protein influences the nucleation of hydroxyapatite crystals and helps regulate mineralization processes. It associates with collagenous and non-collagenous components in the bone and contributes to tissue integrity and repair. BSP functions as part of a complex matrix that orchestrates cell adhesion and signal transduction impacting osteoblast and osteoclast activity.

Pathways

Bone Sialoprotein interacts within the extracellular matrix organization pathway and the integrin signaling pathway. In the extracellular matrix pathway BSP plays a role together with proteins like osteopontin also from the SIBLING family to influence cellular adhesion. Through integrin signaling BSP modulates cell-surface interactions particularly affecting the behavior of osteoclasts and osteoblasts and their capacity to maintain bone homeostasis.

Bone Sialoprotein associates with pathological conditions such as osteosarcoma and osteoporosis. In osteosarcoma BSP overexpression correlates with aggressive tumor growth potentially linking it with the tumor protein osteopontin suggesting a marker for malignancy. In osteoporosis dysregulation of BSP expression may affect bone density contributing to weakened bone structure alongside changes in other bone matrix proteins.

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Product protocols

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Target data

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes adhesion and migration of various cells via the alpha-V/beta-3 integrin receptor (ITGAV : ITGB3).

See full target information Bone sialoprotein 2

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Bone Sialoprotein ELISA Kit (IBSP)

Sandwich ELISA - Mouse Bone Sialoprotein ELISA Kit (IBSP) (AB279405)

Sandwich ELISA - Mouse Bone Sialoprotein ELISA Kit (IBSP) (AB279405)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise