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AB284621

Mouse Butyrylcholinesterase ELISA Kit (BCHE)

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Mouse Butyrylcholinesterase ELISA Kit (BCHE) is a single-wash 90-min Simplestep used to quantify Mouse Butyrylcholinesterase (BCHE) with a sensitivity of 7.17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Pseudocholinesterase, Bche

4 Images
Sandwich ELISA - Mouse Butyrylcholinesterase ELISA Kit (BCHE) (AB284621)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Butyrylcholinesterase ELISA Kit (BCHE) (AB284621)

Interpolated concentrations of mouse Butyrylcholinesterase in mouse brain extract, mouse heart extract, and mouse liver extract.

Interpolated concentration of native Butyrylcholinesterase was measured in duplicate at different sample concentrations. Undiluted samples are as follows : mouse brain extract 500 ug/mL, mouse heart extract 500 ug/mL, and mouse liver extract 25 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse Butyrylcholinesterase ELISA Kit (BCHE) (AB284621)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Butyrylcholinesterase ELISA Kit (BCHE) (AB284621)

Interpolated concentrations of mouse Butyrylcholinesterase in serum plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native Butyrylcholinesterase was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 0.25%, plasma (citrate) 0.25%, plasma (EDTA) 0.5%, and plasma (heparin) 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Butyrylcholinesterase ELISA Kit (BCHE) (AB284621)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Butyrylcholinesterase ELISA Kit (BCHE) (AB284621)

Example of mouse Butyrylcholinesterase standard curve in 1X Cell Extraction buffer PTR.

Example of mouse Butyrylcholinesterase standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Butyrylcholinesterase ELISA Kit (BCHE) (AB284621)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Butyrylcholinesterase ELISA Kit (BCHE) (AB284621)

Example of mouse Butyrylcholinesterase standard curve in Sample Diluent NS.

Example of mouse Butyrylcholinesterase standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.17 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Butyrylcholinesterase ELISA kit (ab284621) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Butyrylcholinesterase protein in mouse serum, plasma, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Butyrylcholinesterase with 7.17 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 98 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "94 - 110 %", "average": "= 102" }, { "sample": "Tissue Extracts", "range": "93 - 106 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "91 - 106 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "101 - 105 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284621-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB284621-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB284621-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB284621-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB284621-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB284621-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB284621-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB284621-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB284621-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Butyrylcholinesterase (BCHE) Capture Antibody", "number":"AB284621-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Butyrylcholinesterase (BCHE) Lyophilized Recombinant Protein", "number":"AB284621-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Butyrylcholinesterase (BCHE) Detector Antibody", "number":"AB284621-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB284621-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Butyrylcholinesterase often called BuChE or BChE is a serine hydrolase enzyme that catalyzes the hydrolysis of ester compounds such as butyrylcholine. It has a molecular mass of approximately 85 kDa. BChE is structurally similar to acetylcholinesterase (AChE) and is found in various tissues with high expression in the liver plasma and the central nervous system. This enzyme acts as a scavenger by breaking down circulating choline esters and certain therapeutic drugs playing an important role in detoxifying the blood.
Biological function summary

This enzyme is significant in nervous system function and is not restricted to acetylcholine breakdown but can hydrolyze larger ester molecules. It forms part of a complex regulatory mechanism alongside acetylcholinesterase ensuring precise modulating of neurotransmitter signaling. BChE can also serve as a backup to acetylcholinesterase in breaking down neurotransmitters therefore contributing to maintaining synaptic balance and functionality.

Pathways

BuChE is involved in the cholinergic signaling pathway playing a significant role in neurotransmitter regulation. This pathway is important for muscle activation and memory processing. BChE also impacts the metabolism of certain ester-type drugs used in anesthesia. Its activity balances with acetylcholinesterase modulating the availability of acetylcholine within synaptic clefts and seamlessly integrating into broader physiological processes.

Altered BChE activity correlates with Alzheimer's disease and certain liver diseases. In Alzheimer's decreased BChE activity parallels reduced acetylcholinesterase function which affects cholinergic transmission in the brain contributing to memory deficits. Disorders like liver cirrhosis also show a compromised BChE level due to liver's impaired enzyme production aligning it closely with liver function and disease diagnostics.
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Product protocols

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Target data

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
See full target information Bche
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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