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AB203361

Mouse c-Kit ELISA Kit (CD117)

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Mouse c-Kit ELISA Kit (CD117) is a single-wash 90-min Simplestep used to quantify Mouse c-Kit (CD117) with a sensitivity of 30 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD117, Sl, Kit, Mast/stem cell growth factor receptor Kit, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit

4 Images
Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (AB203361)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (AB203361)

Interpolated concentrations of c-Kit in mouse plasma (EDTA, heparin and citrate).

The concentrations of c-Kit were measured in duplicate and interpolated from the c-Kit standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (AB203361)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (AB203361)

Example of c-KIT standard curve prepared in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (AB203361)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (AB203361)

Interpolated concentrations of c-Kit in mouse brain and lung extract.

The concentrations of c-Kit were measured in duplicate and interpolated from the c-Kit standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Interpolated values of c-Kit are plotted based on an extract load of 200 μg/mL.

Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (AB203361)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (AB203361)

Example of c-KIT standard curve prepared in 1X Cell Extraction buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 30 pg/mL

Range

0.23 - 15 ng/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Complementary Products

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse c-Kit ELISA Kit (CD117) (ab203361) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of c-Kit (CD117) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse c-Kit (CD117) with 30 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

c-Kit (CD117) is a member of the type III subfamily of receptor tyrosine kinases and is the cellular receptor for stem cell factor. Kit is essential for cell survival and proliferation, stem cell maintenance, mast cell development, melanogenesis, cellular migration, hematopoiesis, and gametogenesis. Mutations in human c-Kit are associated with gastrointestional stromal tumors, piebaldism, acute myelogenous leukemia, and mast cell disease.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "92 - 94 %", "average": "= 94" }, { "sample": "Serum", "range": "115 - 120 %", "average": "= 118" }, { "sample": "EDTA Plasma", "range": "93 - 144 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "104 - 114 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "103 - 119 %", "average": "= 110" }, { "sample": "Mouse brain extract", "range": "103 - 106 %", "average": "= 104" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Kit protein also known as CD117 is a type of receptor tyrosine kinase with a molecular weight of approximately 145 kDa. It is encoded by the KIT gene and commonly expressed on the surface of hematopoietic stem cells mast cells melanocytes and germ cells. C-Kit plays a role in the signal transduction pathways that control cell proliferation and survival. Researchers often identify c-Kit using markers like biotin c-Kit PerCP Cy5.5 or antibodies such as anti-c-Kit for experimental purposes.
Biological function summary

C-Kit influences the function and survival of certain cell types. When it binds to its ligand stem cell factor (SCF) it activates receptor dimerization and phosphorylation triggering downstream signaling. C-Kit does not act alone; it often functions in a complex with other proteins to affect cellular outcomes. Its expression is vital for the maintenance of bone marrow hematopoietic cells and contributes to melanocyte and germ cell development.

Pathways

C-Kit interacts with the PI3K/AKT and RAS/ERK pathways through its kinase activity. These pathways play important roles in cell division differentiation and survival. The c-Abl and ACK2 proteins are closely related to c-Kit function within these signaling networks contributing to regulating cellular responses to external stimuli.

C-Kit mutations are frequently associated with gastrointestinal stromal tumors (GIST) and acute myeloid leukemia (AML). These conditions often involve alterations in c-Kit activity leading to uncontrolled cell growth. While studying GIST researchers have noticed interactions between c-Kit and other proteins like CBL which is involved in the modulation of c-Kit signaling. Understanding these interactions helps in designing targeted therapies for conditions involving c-Kit dysregulation.
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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
See full target information Kit
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websiteProtocolBooklet
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Product promise

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