Mouse C-Peptide ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse C-Peptide in Serum, Citrate plasma, EDTA Plasma, Cell culture media samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 3.516 - 225 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.16 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Alternative names
Ins-1, Ins1, Insulin-1
What's included?
Mouse C-Peptide ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse C-Peptide in Serum, Citrate plasma, EDTA Plasma, Cell culture media samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 3.516 - 225 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.16 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. = 7.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. = 5.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 103 | Range 102 - 107 % |
Sample type Citrate plasma | Average % = 93 | Range 91 - 95 % |
Sample type EDTA Plasma | Average % = 95 | Range 91 - 97 % |
Sample type Cell culture media | Average % = 89 | Range 88 - 90 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse C-Peptide SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C-Peptide protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media. Quantitate Mouse C-Peptide with 1.16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Singlewash protocol reduces assay time to 90 minutes or less.
- High sensitivity, specificity and reproducibility from superior antibodies.
- Fully validated in biological samples.
- 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Mouse C-Peptide ELISA Kit (ab324138)
Example of mouse C-Peptide standard curve in 1X Sample Diluent PT. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse C-Peptide ELISA Kit (ab324138)
Interpolated concentration of native C-Peptide was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 50%, plasma (citrate) 25%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Sample Diluent PT.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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