Mouse C Reactive Protein ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse C Reactive Protein with a sensitivity of 17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- International journal of molecular sciences 24:2023Magnetic Nanoemulsions for the Intra-Articular Delivery of Ascorbic Acid and Dexamethasone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamelia Mihaela Zară-Dănceanu,Cristina Stavilă,Anca Emanuela Minuti,Luminiţa Lăbușcă,Valentin Nastasa,Dumitru-Daniel Herea,Răzvan-Nicolae Malancus,Daniel Ghercă,Sorin-Aurelian Pasca,Horia Chiriac,Mihai Mares,Nicoleta LupuPubMed 37569290
- Journal of cellular and molecular medicine 27:2228-22382023Over-expression of Dyrk1A affects bleeding by modulating plasma fibronectin and fibrinogen level in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuillaume Postic,Jean Solarz,Cécile Loubière,Janany Kandiah,Jaysen Sawmynaden,Frederic Adam,Marie Vilaire,Thibaut Léger,Jean-Michel Camadro,Daniella Balduino Victorino,Marie-Claude Potier,Eric Bun,Gautier Moroy,Alexandre Kauskot,Olivier Christophe,Nathalie JanelPubMed 37415307
- Frontiers in nutrition 9:10602122023Surfeit folic acid, protein, and exercise modify oncogenic inflammatory biomarkers and fecal microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca M Tuska,Sophia M Helm,C Foster Graf,Courtney James,Gabriel Kong,Leah T Stiemsma,David B Green,Susan Edgar HelmPubMed 36742002
- Evidence-based complementary and alternative medicine : eCAM 2022:25701692022The Clinical Effect and Mechanism of Prostant on Urinary Retention and Anal Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Min Luo,Han Du,Hong-Liang Jiang,Ying-Jun Deng,Xue Liang,Ping Qiu,Yao ChengPubMed 36110189
- Biology 11:2022Low-Salt Diet Reduces Anti-CTLA4 Mediated Systemic Immune-Related Adverse Events while Retaining Therapeutic Efficacy against Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDurga Khandekar,Debolanle O Dahunsi,Isaac V Manzanera Esteve,Sonya Reid,Jeffrey C Rathmell,Jens Titze,Venkataswarup TiriveedhiPubMed 35741331
- Mediators of inflammation 2021:99790322021Recombinant Human Thymosin Beta-4 Protects against Mouse Coronavirus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Yu,Yunyun Mao,Kai Li,Yanfang Zhai,Yue Zhang,Shuling Liu,Yuemei Gao,Zhengshan Chen,Yanhong Liu,Ting Fang,Mengsu Zhao,Ruihua Li,Junjie Xu,Wei ChenPubMed 33967626
- Tissue engineering. Part C, Methods 26:67-792020A Comparison of Sterilization Techniques for Production of Decellularized Intestine in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolyn Gosztyla et. al.PubMed 31802699
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 17 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Alternative names
Ptx1, Crp, C-reactive protein
What's included?
Recommended products
Mouse C Reactive Protein ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse C Reactive Protein with a sensitivity of 17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 17 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 5 | C.V. 2.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 2.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111 | Range 109 - 113 % |
Sample type EDTA Plasma | Average % = 107 | Range 103 - 109 % |
Sample type Tissue Extracts | Average % = 115 | Range 103 - 122 % |
Sample type Heparin Plasma | Average % = 113 | Range 110 - 117 % |
Sample type Citrate plasma | Average % = 108 | Range 90 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Mouse C Reactive Protein ELISA Kit ab222511 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse C Reactive Protein in citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 17 pg/mL.
How the assay works
Mouse C Reactive Protein SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse C Reactive Protein ELISA Kit ab222511 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-C Reactive Protein antibody [EPR18538-23] - BSA and Azide free (Capture) Anti-C Reactive Protein antibody [EPR18536-15] - BSA and Azide free (Detector) ab242938
- Anti-C Reactive Protein antibody [EPR18536-15] - BSA and Azide free (Detector) Anti-C Reactive Protein antibody [EPR18536-15] - BSA and Azide free (Detector) ab242938
C Reactive Protein displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. C Reactive Protein can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. C Reactive Protein is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. C Reactive Protein binds 2 calcium ions per subunit. The concentration of C Reactive Protein in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1and IL6//interleukin-6.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
- Biological function summary
CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.
- Pathways
CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.
- Associated diseases and disorders
Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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ELISA - Mouse C Reactive Protein ELISA Kit (ab222511)
ELISA - Mouse C Reactive Protein ELISA Kit (ab222511)
Example of mouse C Reactivte Protein standard curve in Sample Diluent NS + Enhancer.
The C Reactive Protein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Mouse C Reactive Protein ELISA Kit (ab222511)
Example of mouse C Reactive Protein standard curve in 1X Cell Extraction Buffer PTR.
The C Reactive Protein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Mouse C Reactive Protein ELISA Kit (ab222511)
Interpolated concentrations of native C Reactive Protein in mouse serum and plasma samples.
The concentrations of C Reactive Protein were measured in duplicates, interpolated from the C Reactive Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:800, plasma (heparin) 1:400, and plasma (EDTA) 1:1600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C Reactive Protein concentration was determined to be 2854 ng/mL in neat serum, 5469 ng/mL in neat plasma (citrate), 3358 ng/mL in neat plasma (heparin), and 7251 ng/mL in neat plasma (EDTA).
ELISA - Mouse C Reactive Protein ELISA Kit (ab222511)
Interpolated concentrations of native C Reactive Protein in mouse liver tissue extract.
Based on a 200 μg/mL extract load and mouse heart tissue extract based on a 500 μg/mL extract load. The concentrations of C Reactive Protein were measured in duplicate and interpolated from the C Reactive Protein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C Reactive Protein concentration was determined to be 7416 pg/mL in mouse liver tissue extract and 5978 pg/mL in mouse heart tissue extract.
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