Mouse C3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse C3 with a sensitivity of 25.52 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Cellular & molecular biology letters 28:792023Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuimeng Qi,Zhaoguo Zheng,Qiang LiuPubMed 37828427
- Journal of virology 97:e00556232023Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGi Uk Jeong,Sumin Lee,Do Yeon Kim,Jaemyun Lyu,Gun Young Yoon,Kyun-Do Kim,Keun Bon Ku,Junsu Ko,Young-Chan KwonPubMed 37191498
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 25.52 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. The short isoform has B-cell stimulatory activity. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2.
Alternative names
Complement C3, HSE-MSF, C3
What's included?
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Mouse C3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse C3 with a sensitivity of 25.52 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 25.52 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 110 | Range 108 - 112 % |
Sample type Serum | Average % = 111 | Range 107 - 113 % |
Sample type EDTA Plasma | Average % = 112 | Range 101 - 119 % |
Sample type Cell culture media | Average % = 95 | Range 94 - 96 % |
Sample type Tissue Extracts | Average % = 111 | Range 106 - 115 % |
Sample type Heparin Plasma | Average % = 105 | Range 94 - 116 % |
Sample type Citrate plasma | Average % = 111 | Range 106 - 114 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse C3 ELISA Kit (ab263884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C3 protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse C3 with 25.52 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
C3, often known as complement component 3, is a protein part of the immune system where it plays a central role in the activation of the complement system. C3 is mainly synthesized in the liver and found to be the most abundant complement protein in serum. C3 is comprised of alpha and beta chains connected covalently by a single disulfide bond and associated by non-covalent forces. Studies have shown people with C3 deficiency are susceptible to bacterial infection. Standard protein in this product is the C3c alpha chain fragment 2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.
- Biological function summary
Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.
- Pathways
The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.
- Associated diseases and disorders
Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.
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4 product images
Sandwich ELISA - Mouse C3 ELISA Kit (ab263884)
Example of mouse C3 standard curve in Sample Diluent NS + Enhancer.
The C3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse C3 ELISA Kit (ab263884)
Interpolated concentrations of native C3 in mouse serum and plasma samples.
The concentrations of C3 were measured in duplicates, interpolated from the C3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:133,333, plasma (citrate) 1:100,000, plasma (EDTA) 1:100,000, and plasma (heparin) 1:133,333. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C3 concentration was determined to be 172.6 μg/mL in serum, 117.6 μg/mL in plasma (citrate), 234.6 μg/mL in plasma (EDTA), and 410.8 μg/mL in plasma (heparin).
Sandwich ELISA - Mouse C3 ELISA Kit (ab263884)
Interpolated concentrations of native C3 in mouse kidney and liver tissue extracts based on a 10 ng/mL extract load respectively.
The concentrations of C3 were measured in duplicate and interpolated from the C3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C3 concentration was determined to be 3,036 pg/mL in kidney tissue and 2,949 pg/mL in liver tissue.
Sandwich ELISA - Mouse C3 ELISA Kit (ab263884)
Interpolated concentrations of native C3 in mouse tissue culture supernatant sample.
The concentrations of C3 were measured in duplicates, interpolated from the C3 standard curves and corrected for sample dilution. Undiluted samples are as follows: liver supernatant 1:80. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C3 concentration was determined to be 207.0 ng/mL liver supernatant.
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