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AB284624

Mouse Calreticulin ELISA Kit

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Mouse Calreticulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Calreticulin with a sensitivity of 49 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, ERp60, Calr

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Sandwich ELISA - Mouse Calreticulin ELISA Kit (AB284624)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Calreticulin ELISA Kit (AB284624)

Example of mouse Calreticulin standard curve in 1X Cell Extraction Buffer PTR.

Example of mouse Calreticulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Calreticulin ELISA Kit (AB284624)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Calreticulin ELISA Kit (AB284624)

Interpolated concentrations of mouse Calreticulin in NIH3T3 cell extract.

Interpolated concentration of native Calreticulin was measured in duplicate at different sample concentrations. Undiluted samples are 200 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 49 pg/mL

Range

140.6 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Calreticulin ELISA kit (ab284624) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Calreticulin protein in mouse cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Calreticulin with 49 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "86 - 119 %", "average": "= 96" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284624-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB284624-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB284624-CMP15", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB284624-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB284624-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Calreticulin Lyophilized Recombinant Protein", "number":"AB284624-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Calreticulin Capture Antibody", "number":"AB284624-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Calreticulin Detector Antibody", "number":"AB284624-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB284624-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB284624-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB284624-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB284624-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB284624-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed : 20880849, PubMed : 21652723). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (By similarity). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
See full target information Calr
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websiteProtocolBooklet
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Product promise

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