AB119585

Mouse Cathepsin B ELISA Kit

Name: Mouse Cathepsin B ELISA Kit (ab119585) | Abcam
Brand: Abcam Limited
SKU: AB119585
Price: 780 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Mouse Cathepsin B ELISA Kit is a sandwich ELISA designed to quantify Mouse Cathepsin B with a sensitivity of 5 pg/well.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 156 - 10000 pg/well

View Alternative Names

Cathepsin B, Cathepsin B1, Ctsb

1 Images

Sandwich ELISA - Mouse Cathepsin B ELISA Kit (AB119585)

sELISA

Supplier Data

Sandwich ELISA - Mouse Cathepsin B ELISA Kit (AB119585)

Representative Standard Curve Using ab119585.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 5 pg/well

Range

156 - 10000 pg/well

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Cathepsin B ELISA Kit ab119585 is a sandwich ELISA to measure Mouse Cathepsin B in serum, EDTA plasma, heparin plasma, cell culture supernatant, cell lysate with a sensitivity of 5 pg/well.

How the assay works

A Cathepsin B specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cathepsin B amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Cathepsin B ELISA Kit ab119585 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Add prepared biotin antibody to each well. Incubate at room temperature.
3. Add prepared Avidin-Biotin-Peroxidase Complex (ABC).
4. Incubate at room temperature.
5. Add TMB to each well. Incubate at room temperature.
6. Add Stop Solution to each well. Read.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "229 pg/mL", "standardDeviation": "13.96", "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1916 pg/mL", "standardDeviation": "128.37", "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4534 pg/mL", "standardDeviation": "439.79", "coefficientOfVariability": "9.7" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "242 pg/mL", "standardDeviation": "14.76", "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1828 pg/mL", "standardDeviation": "100.54", "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4749 pg/mL", "standardDeviation": "370.42", "coefficientOfVariability": "7.8" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119585-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119585-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse Cathepsin B antibody", "number":"AB119585-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse Cathepsin B antibody Microplate (12 x 8 wells)", "number":"AB119585-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119585-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119585-CMP09", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB119585-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119585-CMP07", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse Cathepsin B standard", "number":"AB119585-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119585-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119585-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119585-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.

Biological function summary

Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.

Pathways

Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.

Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.

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Product protocols

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Target data

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (By similarity). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (PubMed : 12782676). Has also been implicated in tumor invasion and metastasis (By similarity).

See full target information CATB_MOUSE

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:1096 PubMed38321014

2024

Pathogenic NLRP3 mutants form constitutively active inflammasomes resulting in immune-metabolic limitation of IL-1β production.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Molina-López,Laura Hurtado-Navarro,Carlos J García,Diego Angosto-Bazarra,Fernando Vallejo,Ana Tapia-Abellán,Joana R Marques-Soares,Carmen Vargas,Segundo Bujan-Rivas,Francisco A Tomás-Barberán,Juan I Arostegui,Pablo Pelegrin

Cell reports 42:113436 PubMed37952157

2023

Skeletal muscle TFEB signaling promotes central nervous system function and reduces neuroinflammation during aging and neurodegenerative disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Matthews,Allison Birnbaum,Anastasia Gromova,Amy W Huang,Kailin Liu,Eleanor A Liu,Kristen Coutinho,Megan McGraw,Dalton C Patterson,Macy T Banks,Amber C Nobles,Nhat Nguyen,Gennifer E Merrihew,Lu Wang,Eric Baeuerle,Elizabeth Fernandez,Nicolas Musi,Michael J MacCoss,Helen C Miranda,Albert R La Spada,Constanza J Cortes

Frontiers in molecular neuroscience 16:1170313 PubMed37138705

2023

Peroxisomal defects in microglial cells induce a disease-associated microglial signature.

Applications

Unspecified application

Species

Unspecified reactive species

Quentin Raas,Ali Tawbeh,Mounia Tahri-Joutey,Catherine Gondcaille,Céline Keime,Romain Kaiser,Doriane Trompier,Boubker Nasser,Valerio Leoni,Emma Bellanger,Maud Boussand,Yannick Hamon,Alexandre Benani,Francesca Di Cara,Caroline Truntzer,Mustapha Cherkaoui-Malki,Pierre Andreoletti,Stéphane Savary

Autophagy 18:2036-2049 PubMed34964698

2021

DCN released from ferroptotic cells ignites AGER-dependent immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Liu,Shan Zhu,Ling Zeng,Jingbo Li,Daniel J Klionsky,Guido Kroemer,Jianxin Jiang,Daolin Tang,Rui Kang

Investigative ophthalmology & visual science 60:1328-1335 PubMed30933260

2019

Low-Intensity Exercise in Mice Is Sufficient to Protect Retinal Function During Light-Induced Retinal Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas M Mees,Monica M Coulter,Micah A Chrenek,Cara T Motz,Erica G Landis,Jeffrey H Boatright,Machelle T Pardue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Cathepsin B ELISA Kit

Sandwich ELISA - Mouse Cathepsin B ELISA Kit (AB119585)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise