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Mouse Cathepsin B ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Cathepsin B in Serum, Citrate plasma, Cell culture media samples.

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Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, Cell culture media

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

28.125 - 1800 pg/mL

Assay time

1h 30m

Sensitivity

= 11.81 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (By similarity). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (PubMed:12782676). Has also been implicated in tumor invasion and metastasis (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Mouse Cathepsin B Capture Antibody
1 x 600 µL
10X Mouse Cathepsin B Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BR
1 x 6 mL
Mouse Cathepsin B Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Mouse Cathepsin B ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Cathepsin B in Serum, Citrate plasma, Cell culture media samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, Cell culture media

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

28.125 - 1800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 11.81 pg/mL

General recovery

110%

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

7.6

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

10

Recovery

Sample specific recovery

Sample type

Serum

Average %
Range

104 - 116 %

Sample type

Citrate plasma

Average %
Range

80 - 101 %

Sample type

Cell culture media

Average %
Range

99 - 104 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse Cathepsin B SimpleStep ELISA® kit (ab303743) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Cathepsin B protein in mouse serum, Cit plasma and cell culture media. Quantitate Mouse Cathepsin B with 11.81 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Mouse Cathepsin B ELISA Kit (ab303743), expandable thumbnail

    Sandwich ELISA - Mouse Cathepsin B ELISA Kit (ab303743)

    Interpolated concentration of native Cathepsin B was measured in duplicate at different sample concentrations. Undiluted samples are 1: 2,000 serum and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Mouse Cathepsin B ELISA Kit (ab303743), expandable thumbnail

    Sandwich ELISA - Mouse Cathepsin B ELISA Kit (ab303743)

    Example of mouse Cathepsin B standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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