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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse Cathepsin B ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Cathepsin B in Serum, Citrate plasma, Cell culture media samples.
Colorimetric
Serum, Citrate plasma, Cell culture media
Sandwich (quantitative)
Mouse
28.125 - 1800 pg/mL
1h 30m
= 11.81 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (By similarity). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (PubMed:12782676). Has also been implicated in tumor invasion and metastasis (By similarity).
Cathepsin B, Cathepsin B1, Ctsb
Mouse Cathepsin B ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Cathepsin B in Serum, Citrate plasma, Cell culture media samples.
Cathepsin B, Cathepsin B1, Ctsb
Colorimetric
Serum, Citrate plasma, Cell culture media
Sandwich (quantitative)
Mouse
28.125 - 1800 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 11.81 pg/mL
110%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 7.6 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 10 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % | Range 104 - 116 % |
Sample type Citrate plasma | Average % | Range 80 - 101 % |
Sample type Cell culture media | Average % | Range 99 - 104 % |
Blue Ice
+4°C
+4°C
+4°C
Mouse Cathepsin B SimpleStep ELISA® kit (ab303743) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Cathepsin B protein in mouse serum, Cit plasma and cell culture media. Quantitate Mouse Cathepsin B with 11.81 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Interpolated concentration of native Cathepsin B was measured in duplicate at different sample concentrations. Undiluted samples are 1: 2,000 serum and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Example of mouse Cathepsin B standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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