AB303743

Mouse Cathepsin B ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Mouse Cathepsin B ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Cathepsin B with a sensitivity of 11.81 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cathepsin B, Cathepsin B1, Ctsb

2 Images

Sandwich ELISA - Mouse Cathepsin B ELISA Kit (AB303743)

sELISA

Supplier Data

Sandwich ELISA - Mouse Cathepsin B ELISA Kit (AB303743)

Interpolated concentrations of mouse Cathepsin B in serum and plasma (citrate)

Interpolated concentration of native Cathepsin B was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 2,000 serum and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Cathepsin B ELISA Kit (AB303743)

Example of mouse Cathepsin B standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, Cell culture media

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.81 pg/mL

Range

28.125 - 1800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse Cathepsin B SimpleStep ELISA® kit (ab303743) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Cathepsin B protein in mouse serum, Cit plasma and cell culture media. Quantitate Mouse Cathepsin B with 11.81 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "104 - 116 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "80 - 101 %", "average": "= 90" }, { "sample": "Cell culture media", "range": "99 - 104 %", "average": "= 102" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303743-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303743-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303743-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303743-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303743-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303743-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Cathepsin B Capture Antibody", "number":"AB303743-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Cathepsin B Lyophilized Recombinant Protein", "number":"AB303743-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB303743-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB303743-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Cathepsin B Detector Antibody", "number":"AB303743-CMP03", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.

Biological function summary

Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.

Pathways

Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.

Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.

style

left-column

Product protocols

style

left-column

Target data

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (By similarity). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (PubMed : 12782676). Has also been implicated in tumor invasion and metastasis (By similarity).

See full target information CATB_MOUSE

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:113436 PubMed37952157

2023

Skeletal muscle TFEB signaling promotes central nervous system function and reduces neuroinflammation during aging and neurodegenerative disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Matthews,Allison Birnbaum,Anastasia Gromova,Amy W Huang,Kailin Liu,Eleanor A Liu,Kristen Coutinho,Megan McGraw,Dalton C Patterson,Macy T Banks,Amber C Nobles,Nhat Nguyen,Gennifer E Merrihew,Lu Wang,Eric Baeuerle,Elizabeth Fernandez,Nicolas Musi,Michael J MacCoss,Helen C Miranda,Albert R La Spada,Constanza J Cortes

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Cathepsin B ELISA Kit

Sandwich ELISA - Mouse Cathepsin B ELISA Kit (AB303743)

Sandwich ELISA - Mouse Cathepsin B ELISA Kit (AB303743)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise