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AB213845

Mouse Cathepsin D ELISA Kit

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(1 Publication)

Mouse Cathepsin D ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Cathepsin D in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Cathepsin D, Ctsd

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Sandwich ELISA - Mouse Cathepsin D ELISA Kit (AB213845)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Cathepsin D ELISA Kit (AB213845)

Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

312 - 20000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse Cathepsin D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213845) is designed for the quantitative measurement of Mouse Cathepsin D in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Cathepsin D has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: I21-L410) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cathepsin D amount of sample captured in plate.

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1778 pg/mL", "standardDeviation": "99.56", "coefficientOfVariability": "= 5.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "844 pg/mL", "standardDeviation": "63.3", "coefficientOfVariability": "= 7.5" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1799 pg/mL", "standardDeviation": "133.12", "coefficientOfVariability": "= 7.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "8034 pg/mL", "standardDeviation": "514.17", "coefficientOfVariability": "= 6.4" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "7768 pg/mL", "standardDeviation": "458.31", "coefficientOfVariability": "= 5.9" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "898 pg/mL", "standardDeviation": "77.43", "coefficientOfVariability": "= 8.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213845-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213845-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Mouse Cathepsin D antibody", "number":"AB213845-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse Cathepsin D coated Microplate (12 x 8 wells)", "number":"AB213845-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213845-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213845-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213845-CMP03", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213845-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Mouse Cathepsin D standard", "number":"AB213845-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213845-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213845-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213845-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin D also known as CTSD is a protein with a mass of approximately 45 kDa. It functions as an aspartyl protease and is expressed in lysosomes across various tissues including the liver and kidneys. This enzyme acts by cleaving peptide bonds within proteins which is essential for protein degradation and turnover. Cathepsin D exists as precursor forms that become activated in the acidic environment of the lysosome. It plays a critical role in normal cellular processes by maintaining protein homeostasis.
Biological function summary

The enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.

Pathways

Cathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.

The overexpression of Cathepsin D links to breast cancer and Alzheimer's disease. In breast cancer increased Cathepsin D expression correlates with tumor progression and metastasis influencing tumor behavior through interactions with other proteins involved in cell proliferation. Alzheimer's disease features the involvement of Cathepsin D in the breakdown of amyloid precursor protein which relates to amyloid beta plaque accumulation. The abnormal activity of Cathepsin D in these disorders makes it a potential target for therapeutic antibodies such as CTSD antibodies which aim to regulate its function.
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Product protocols

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Target data

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation.
See full target information Ctsd
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:4643 PubMed34330919

2021

Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Martinelli,Elmira A Anderzhanova,Thomas Bajaj,Svenja Wiechmann,Frederik Dethloff,Katja Weckmann,Daniel E Heinz,Tim Ebert,Jakob Hartmann,Thomas M Geiger,Michael Döngi,Kathrin Hafner,Max L Pöhlmann,Lee Jollans,Alexandra Philipsen,Susanne V Schmidt,Ulrike Schmidt,Giuseppina Maccarrone,Valentin Stein,Felix Hausch,Christoph W Turck,Mathias V Schmidt,Anne-Kathrin Gellner,Bernhard Kuster,Nils C Gassen
View all publications
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websiteProtocolBooklet
en
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