Mouse Cathepsin D ELISA Kit
- Recombinant
- SimpleStep
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(4 Publications)
Mouse Cathepsin D ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Cathepsin D with a sensitivity of 775 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Cathepsin D, Ctsd
- sELISA
Unknown
Sandwich ELISA - Mouse Cathepsin D ELISA Kit (AB239420)
Example of mouse Cathepsin D standard curve in Sample Diluent NS. The Cathepsin D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Mouse Cathepsin D ELISA Kit (AB239420)
Interpolated concentrations of native Cathepsin D in mouse serum and plasma samples.
The concentrations of Cathepsin D were measured in duplicate, interpolated from the Cathepsin D standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (Heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 366.2 ng/mL in serum, 300.8 ng/mL in plasma (citrate), 213.9 ng/mL in plasma (EDTA), and 446.9 ng/mL in plasma (Heparin).
Reactivity data
Product details
Mouse Cathepsin D ELISA Kit (ab239420) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin D protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Cathepsin D with 775 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Recovery
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.
Pathways
Cathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.
Product protocols
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Target data
Publications (4)
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Cells 12: PubMed37681871
2023
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Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 56:150-165 PubMed35413750
2022
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Journal of cellular and molecular medicine 24:7907-7914 PubMed32529705
2020
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Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 54:287-302 PubMed32246616
2020
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