AB202406

Mouse CCL27 ELISA Kit (CTACK)

Recombinant
SimpleStep
What is this?

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Mouse CCL27 ELISA Kit (CTACK) is a single-wash 90-min Simplestep used to quantify Mouse CCL27 (CTACK) with a sensitivity of 9.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ilc, Scya27, Ccl27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK, ALP, mILC

3 Images

Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)

sELISA

Supplier Data

Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)

CCL27 levels in stimulated (1.5% PHA + 10 ng/mL PMA, 72 hrs (+)) and unstimulated (-) mouse L929 supernatants.

A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 100% for both the stimulated and unstimulated supernatants. Interpolated data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)

Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.

The 1X dilution was 3.13% for mouse serum, 6.25% for citrate and EDTA plasma, and 1.56% for heparin plasma. Interpolated data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)

Example of CCL27 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 9.3 pg/mL

Range

23.4 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse CCL27 ELISA Kit (CTACK) (ab202406) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL27 (CTACK) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL27 (CTACK) with 9.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CCL27 is a chemotactic factor that attracts skin-associated memory T-lymphocytes, and binds to CCR10. It may play a role in cell migration during embryogenesis, and may mediate lymphocyte homing to cutaneous sites. Nuclear forms of CCL27 may facilitate cellular migration by inducing cytoskeletal relaxation. There is evidence that CCL27 is strongly upregulated in atopic dermatitis, contact dermatitis, and psoriasis skin lesions.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.94" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.34" } ]

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Recovery

[ { "sample": "Serum", "range": "100 - 116 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "105 - 146 %", "average": "= 128" }, { "sample": "Heparin Plasma", "range": "71 - 114 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "81 - 124 %", "average": "= 107" }, { "sample": "Cell culture media", "range": "103 - 128 %", "average": "= 116" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.

Biological function summary

CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.

Pathways

The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.

CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.

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Product protocols

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Target data

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis. Nuclear forms may facilitate cellular migration by inducing cytoskeletal relaxation. Binds to CCR10.

See full target information Ccl27

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse CCL27 ELISA Kit (CTACK)

Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)

Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)

Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise