Mouse CCL28 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CCL28 with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemotactic for resting CD4, CD8 T-cells and eosinophils (By similarity). Binds to CCR10 and induces calcium mobilization in a dose-dependent manner.
Alternative names
Scya28, Ccl28, C-C motif chemokine 28, Small-inducible cytokine A28
What's included?
Recommended products
Mouse CCL28 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CCL28 with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.7 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Saliva | n 8 | mean - | SD - | C.V. 2.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Saliva | n 3 | mean - | SD - | C.V. 6.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 116 | Range 115 - 118 % |
Sample type Serum | Average % = 111 | Range 99 - 118 % |
Sample type Citrate plasma | Average % = 99 | Range 94 - 105 % |
Sample type EDTA Plasma | Average % = 109 | Range 105 - 112 % |
Sample type Heparin Plasma | Average % = 99 | Range 96 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse CCL28 ELISA Kit (ab210578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL28 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL28 with 4.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse CCL28 is a member of the CC subfamily of chemokines. Mucosal epithelial cells such as exocrine glands, trachea, and colon express mouse CCL28 protein. In lower levels, mouse CCL28 is also found in brain, spleen and lung tissues. CCL28 chemoattracts resting CD4 and CD8 cells, as well as IgA antibody secreting cells. Furthermore, CCL28 plays a role in extravasation of IgA antibody secreting cells in the small intestine, colon, and lactating mammary gland. Human and mouse CCL28 share an overall 66% amino acid sequence identity, with an 83% amino acid sequence identity in their mature regions.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL28 also known as MEC (Mucosae-associated epithelial chemokine) is a chemokine weighing around 12 kDa. It plays a mechanical role in mediating cell chemotaxis promoting the migration of cells towards it. This chemokine predominantly expresses in mucosal tissues including the salivary glands intestines and respiratory tract epithelia. CCL28 interacts specifically with chemokine receptors CCR10 and CCR3 guiding immune cells to these mucosal surfaces where they perform important immune functions.
- Biological function summary
CCL28 plays an important role in mucosal immunity and is involved in the defense against pathogens in these environments. The chemokine attracts lymphocytes and neutrophils aiding in their positioning at mucosal sites. This activity assists in maintaining a balanced immune response preventing excessive inflammation while ensuring adequate pathogen clearance. CCL28 is not part of a larger protein complex but performs critical functions independently coordinating with other immune mediators for effective response.
- Pathways
The function of CCL28 aligns with its involvement in immune signaling and mucosal defense pathways. The chemokine integrates into the immune response network working closely with other chemokines and cytokines like CCL27 and IL-8. It engages in the chemokine signaling pathway which regulates leukocyte migration and positioning. The interplay between CCL28 and these immune mediators ensures proper protection of mucosal surfaces from potential pathogens.
- Associated diseases and disorders
CCL28 holds significant relevance in inflammatory bowel disease (IBD) and allergic rhinitis. In IBD the chemokine contributes to the infiltration of immune cells into the inflamed gut mucosa where its interaction with other cytokines such as TNF-α exacerbates tissue inflammation. In allergic rhinitis CCL28 participates in recruiting eosinophils to the nasal mucosa often correlating with heightened inflammatory responses in these patients and cooperating with proteins like IL-5 to sustain the allergic reaction.
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5 product images
Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)
Example of mouse CCL28 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)
Example of mouse CCL28 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)
Interpolated concentrations of spiked CCL28 in mouse serum and plasma samples.
The concentrations of CCL28 were measured in duplicates, interpolated from the CCL28 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL28 concentration was determined to be 4,718.7 pg/mL in serum, 4,395.5 pg/mL in plasma (citrate), 4,697.8 pg/mL in plasma (EDTA) and 5,130.8 pg/mL in plasma (heparin).
Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)
Interpolated concentrations of native CCL28 in mouse serum and plasma samples.
The concentrations of CCL28 were measured in duplicates, interpolated from the CCL28 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL28 concentration was determined to be 461.6 pg/mL in serum, 615.2 pg/mL in plasma (citrate), 609.5 pg/mL in plasma (EDTA), and 390.0 pg/mL in plasma (heparin).
Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)
Example of mouse CCL28 standard curve in Sample Diluent 20BP.
Background-subtracted data values (mean +/- SD) are graphed.
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