Mouse CCL6 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse CCL6 in Mouse in Plasma, Cell culture supernatant, Serum samples.
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- Breast cancer research : BCR 22:1032020Sunitinib facilitates metastatic breast cancer spreading by inducing endothelial cell senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenian Wang et. al.PubMed 32993785
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 18 - 1000 pg/mL
- Sensitivity
- = 18 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemotactic factor that attracts mostly macrophage, but it can also attract B cells, CD4(+) lymphocytes and eosinophils.
Alternative names
C10, Scya6, Ccl6, C-C motif chemokine 6, Protein C10, Small-inducible cytokine A6
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Mouse CCL6 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse CCL6 in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 18 - 1000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 18 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 104.2 | Range 97 - 113 % |
Sample type Plasma | Average % = 88.31 | Range 81 - 96 % |
Sample type Cell culture media | Average % = 110.2 | Range 92 - 122 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's CCL6 mouse ELISA Kit (ab193719) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CCL6 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CCL6 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CCL6 present in the samples. The wells are washed and biotinylated anti-mouse CCL6 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CCL6 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL6 also known as chemokine (C-C motif) ligand 6 is a small cytokine involved in immune response signaling. It belongs to the CC chemokine family and has a mass of approximately 12 kDa. CCL6 is mainly expressed in monocytes macrophages and T-cells where it plays a role in mediating cell communication and migration. While research has primarily focused on murine models certain expressions in tissues suggest potential counterparts in humans although human-specific studies remain limited.
- Biological function summary
CCL6 functions as a chemoattractant for eosinophils and monocytes important in inflammation processes. It does not form part of larger protein complexes but operates as an individual chemokine influencing various leukocyte activities. Its activity is essential for directing cells to sites of injury or infection thereby playing a significant role in managing immune responses and promoting tissue repair.
- Pathways
CCL6 plays an important role in the MAPK and NF-kB pathways which regulate inflammatory and immune responses. It functions alongside other essential chemokines like CCL2 (MCP-1) and CCL3 (MIP-1α) contributing to the recruitment and activation of immune cells under inflammatory conditions. This recruitment plays an important role in initiating defense mechanisms and coordinating the body's response to harmful stimuli.
- Associated diseases and disorders
CCL6 has strong associations with inflammatory diseases and autoimmune disorders such as rheumatoid arthritis and multiple sclerosis. In these conditions CCL6 or its murine counterparts along with associated proteins like IL-6 and TNF-alpha contribute to the disease pathology by promoting persistent inflammation and immune cell infiltration. Understanding CCL6's involvement can be critical for developing targeted therapies in managing these chronic disorders.
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2 product images
Sandwich ELISA - Mouse CCL6 ELISA Kit (ab193719)
Typical Standard Curve in Assay Diluent A using ab193719 CCL6 Mouse ELISA Kit.
Sandwich ELISA - Mouse CCL6 ELISA Kit (ab193719)
Typical Standard Curve in Assay Diluent B using ab193719 CCL6 Mouse ELISA Kit.
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Product protocols
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