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AB193719

Mouse CCL6 ELISA Kit

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(1 Publication)

Mouse CCL6 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse CCL6 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

C10, Scya6, Ccl6, C-C motif chemokine 6, Protein C10, Small-inducible cytokine A6

2 Images
Sandwich ELISA - Mouse CCL6 ELISA Kit (AB193719)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB193719)

Typical Standard Curve in Assay Diluent B using ab193719 CCL6 Mouse ELISA Kit.

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB193719)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB193719)

Typical Standard Curve in Assay Diluent A using ab193719 CCL6 Mouse ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 18 pg/mL

Range

18 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CCL6 mouse ELISA Kit (ab193719) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CCL6 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse CCL6 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CCL6 present in the samples. The wells are washed and biotinylated anti-mouse CCL6 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CCL6 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 113 %", "average": "= 104.2" }, { "sample": "Plasma", "range": "81 - 96 %", "average": "= 88.31" }, { "sample": "Cell culture media", "range": "92 - 122 %", "average": "= 110.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193719-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193719-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193719-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse CCL-6 Microplate (12 strips x 8 wells)", "number":"AB193719-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193719-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193719-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB193719-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193719-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CCL-6 Standards (lyophilized)", "number":"AB193719-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Detection Antibody CCL6 (biotinylated anti-mouse CCL6)", "number":"AB193719-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL6 also known as chemokine (C-C motif) ligand 6 is a small cytokine involved in immune response signaling. It belongs to the CC chemokine family and has a mass of approximately 12 kDa. CCL6 is mainly expressed in monocytes macrophages and T-cells where it plays a role in mediating cell communication and migration. While research has primarily focused on murine models certain expressions in tissues suggest potential counterparts in humans although human-specific studies remain limited.
Biological function summary

CCL6 functions as a chemoattractant for eosinophils and monocytes important in inflammation processes. It does not form part of larger protein complexes but operates as an individual chemokine influencing various leukocyte activities. Its activity is essential for directing cells to sites of injury or infection thereby playing a significant role in managing immune responses and promoting tissue repair.

Pathways

CCL6 plays an important role in the MAPK and NF-kB pathways which regulate inflammatory and immune responses. It functions alongside other essential chemokines like CCL2 (MCP-1) and CCL3 (MIP-1α) contributing to the recruitment and activation of immune cells under inflammatory conditions. This recruitment plays an important role in initiating defense mechanisms and coordinating the body's response to harmful stimuli.

CCL6 has strong associations with inflammatory diseases and autoimmune disorders such as rheumatoid arthritis and multiple sclerosis. In these conditions CCL6 or its murine counterparts along with associated proteins like IL-6 and TNF-alpha contribute to the disease pathology by promoting persistent inflammation and immune cell infiltration. Understanding CCL6's involvement can be critical for developing targeted therapies in managing these chronic disorders.
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Product protocols

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Target data

Chemotactic factor that attracts mostly macrophage, but it can also attract B cells, CD4(+) lymphocytes and eosinophils.
See full target information Ccl6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 22:103 PubMed32993785

2020

Sunitinib facilitates metastatic breast cancer spreading by inducing endothelial cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Denian Wang,Fei Xiao,Zhongxue Feng,Min Li,Lingmiao Kong,Luping Huang,Yong'gang Wei,Hongyu Li,Fei Liu,Haili Zhang,Wei Zhang
View all publications
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