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AB277715

Mouse CCL6 ELISA Kit

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Mouse CCL6 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CCL6 with a sensitivity of 0.97 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

C10, Scya6, Ccl6, C-C motif chemokine 6, Protein C10, Small-inducible cytokine A6

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Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)

Interpolated concentrations of native or spike CCL6 in mouse serum, plasma and cell culture supernatant samples.

The concentrations of CCL6 were measured in duplicates, interpolated from the CCL6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 20, plasma (citrate) 1 : 80, plasma (EDTA) 1 : 80, plasma (heparin) 1 : 80, and spleen supernatant 1 : 40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL6 concentration was determined to be 3,747 pg/mL in serum, 14,568 pg/mL in plasma (citrate), 20,533 pg/mL in plasma (EDTA), 18,616 pg/mL in plasma (heparin), and 9,852 pg/mL in spleen supernatant.

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)

Example of mouse CCL6 standard curve in Sample Diluent NS. The CCL6 standard curve was prepared as described in Section 10.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)

Example of mouse CCL6 standard curve in 1X Cell Extraction Buffer PTR.

The CCL6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)

Interpolated concentrations of native CCL6 in mouse spleen extract sample based on a 400 μg/mL extract load.

The concentrations of CCL6 were measured in duplicate and interpolated from the CCL6 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL6 concentration was determined to be 125.6 pg/mL in spleen extract.

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CCL6 ELISA Kit (AB277715)

Representative image of Mouse CCL6 ELISA Kit (ab277715)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Mouse CCL6 Detector Antibody

- Mouse CCL6 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Mouse CCL6 Capture Antibody (Lyophilised)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.97 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CCL6 ELISA kit (ab277715) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse CCL6 protein in mouse serum, plasma, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL6 with .97 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 116 %", "average": "= 110" }, { "sample": "Serum", "range": "112 - 116 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "106 - 118 %", "average": "= 112" }, { "sample": "Tissue Extracts", "range": "92 - 97 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "110 - 117 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "106 - 115 %", "average": "= 110" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277715-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277715-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277715-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"Mouse CCL6 Capture Antibody (Lyophilized)", "number":"AB277715-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277715-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CCL6 Lyophilized Recombinant Protein", "number":"AB277715-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB277715-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CCL6 Detector Antibody", "number":"AB277715-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB277715-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277715-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB277715-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277715-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277715-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL6 also known as chemokine (C-C motif) ligand 6 is a small cytokine involved in immune response signaling. It belongs to the CC chemokine family and has a mass of approximately 12 kDa. CCL6 is mainly expressed in monocytes macrophages and T-cells where it plays a role in mediating cell communication and migration. While research has primarily focused on murine models certain expressions in tissues suggest potential counterparts in humans although human-specific studies remain limited.
Biological function summary

CCL6 functions as a chemoattractant for eosinophils and monocytes important in inflammation processes. It does not form part of larger protein complexes but operates as an individual chemokine influencing various leukocyte activities. Its activity is essential for directing cells to sites of injury or infection thereby playing a significant role in managing immune responses and promoting tissue repair.

Pathways

CCL6 plays an important role in the MAPK and NF-kB pathways which regulate inflammatory and immune responses. It functions alongside other essential chemokines like CCL2 (MCP-1) and CCL3 (MIP-1α) contributing to the recruitment and activation of immune cells under inflammatory conditions. This recruitment plays an important role in initiating defense mechanisms and coordinating the body's response to harmful stimuli.

CCL6 has strong associations with inflammatory diseases and autoimmune disorders such as rheumatoid arthritis and multiple sclerosis. In these conditions CCL6 or its murine counterparts along with associated proteins like IL-6 and TNF-alpha contribute to the disease pathology by promoting persistent inflammation and immune cell infiltration. Understanding CCL6's involvement can be critical for developing targeted therapies in managing these chronic disorders.
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Product protocols

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Target data

Chemotactic factor that attracts mostly macrophage, but it can also attract B cells, CD4(+) lymphocytes and eosinophils.
See full target information Ccl6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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