Mouse CCL9 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
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(1 Publication)
Mouse CCL9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CCL9 with a sensitivity of 1.03 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Mrp2, Scya10, Scya9, Ccl9, C-C motif chemokine 9, CCF18, Macrophage inflammatory protein 1-gamma, Macrophage inflammatory protein-related protein 2, Small-inducible cytokine A9, MIP-1-gamma, MRP-2
- sELISA
Unknown
Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)
Interpolated concentrations of native CCL9 in mouse J774A.1 and RAW264.7 cell culture supernatant samples. The concentrations of CCL9 were measured in duplicates, interpolated from the CCL9 standard curves and corrected for sample dilution. Undiluted samples are as follows : J774A.1 cell culture supernatant 1 : 200 and RAW264.7 cell culture supernatant 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL9 concentration was determined to be 12.5 ng/ml in J774A.1 cell culture supernatant and 34.0 ng/ml in RAW264.7 cell culture supernatant.
- sELISA
Unknown
Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)
Example of mouse CCL9 standard curve in Sample Diluent NS. The CCL9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)
Interpolated concentrations of native CCL9 in mouse serum and plasma samples. The concentrations of CCL9 were measured in duplicates, interpolated from the CCL9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 200, plasma (heparin) 1 : 100, and plasma (EDTA) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL9 concentration was determined to be 12.5 ng/mL in serum, 11.4 ng/mL in plasma (citrate), 13.8 ng/mL in plasma (heparin), and 14.2 ng/ml in plasma (EDTA).
- sELISA
Unknown
Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)
Mouse CCL9 Standard Curve Comparison. Standard Curve comparison between mouse CCL9 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Reactivity data
Product details
Mouse CCL9 ELISA Kit (ab240689) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL9 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL9 with 1.03 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MIP-1γ contributes to immune response modulation by acting as a signaling molecule. It helps coordinate the movement and activation of immune cells such as monocytes and lymphocytes thereby modulating inflammation. Unlike some other chemokines MIP-1γ does not usually form a complex with proteins. Its primary role involves direct interactions with specific receptors on target cells that initiate intracellular signaling pathways leading to cell migration and activation.
Pathways
MIP-1γ engages in chemokine signaling and inflammatory response pathways. It intricately associates with the chemokine receptor CCR1 which is vital for mediating signal transduction processes essential for cell movement and immune surveillance. MIP-1γ also plays a role in the NF-kB signaling pathway important for transcription regulation and inflammatory response. This relationship highlights its involvement in orchestrating immune cell migration and the regulation of immune responses.
Product protocols
- Visit the General protocols
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- Download websiteProtocolBooklet|en
Target data
Publications (1)
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Experimental and therapeutic medicine 23:226 PubMed35222703
2022
Applications
Unspecified application
Species
Unspecified reactive species
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