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AB272204

Mouse CD163 ELISA Kit

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(6 Publications)

Mouse CD163 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CD163 with a sensitivity of 66.43 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

CD163, M130, Cd163, Scavenger receptor cysteine-rich type 1 protein M130

4 Images
ELISA - Mouse CD163 ELISA Kit (AB272204)
  • ELISA

Supplier Data

ELISA - Mouse CD163 ELISA Kit (AB272204)
Sandwich ELISA - Mouse CD163 ELISA Kit (AB272204)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD163 ELISA Kit (AB272204)

Interpolated concentrations of native CD163 in mouse serum and plasma samples.

The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 40, plasma (citrate) 1 : 40, plasma (heparin) 1 : 40, and plasma (EDTA) 1 : 20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 163.26 ng/mL in serum, 171.75 ng/mL in plasma (citrate), 147.88 ng/mL in plasma (heparin), and 127.94 ng/ml in plasma (EDTA).

Sandwich ELISA - Mouse CD163 ELISA Kit (AB272204)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD163 ELISA Kit (AB272204)

Example of mouse CD163 standard curve in Sample Diluent NS.

The CD163 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CD163 ELISA Kit (AB272204)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD163 ELISA Kit (AB272204)

Interpolated concentrations of native CD163 in mouse lung tissue culture supernatant samples treated for three days with 10 µg/mL Concanavalin A.

The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 2,878.13 pg/mL in lung supernatant treated with Concanavalin A.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 66.43 pg/mL

Range

109.38 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CD163 ELISA Kit ab272204 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse CD163 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 66.43 pg/mL.

How the assay works

Mouse CD163 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse CD163 ELISA Kit ab272204 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD163 antibody [EPR22983-47] - BSA and Azide free (Capture) ab272407
- Anti-CD163 antibody [EPR22983-29] - BSA and Azide free (Detector) ab272407

CD163 is a scavenger receptor for the hemoglobin-haptoglobin complex. It is also a receptor for gram-positive and gram-negative bacteria, and a monocyte/macrophage lineage marker. Shedding of CD163 in humans is upregulated in a variety of inflammatory diseases, including rheumatoid arthritis, sepsis, HIV infection and more. This shedding does not occur in mouse CD163.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 117 %", "average": "= 102" }, { "sample": "Serum", "range": "101 - 105 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "91 - 95 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "93 - 96 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "98 - 104 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272204-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272204-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272204-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272204-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272204-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272204-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB272204-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD163 Capture Antibody", "number":"AB272204-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CD163 Lyophilized Recombinant Protein", "number":"AB272204-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272204-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD163 Detector Antibody", "number":"AB272204-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
Biological function summary

CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.

Pathways

CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.

CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.
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Product protocols

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Target data

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity).. After shedding, the soluble form (sCD163) may play an anti-inflammatory role.
See full target information Cd163
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1196704 PubMed37215106

2023

Epigenetically altered macrophages promote development of diabetes-associated atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Huang,Wei Gao,Xin Zhong,Hongxian Wu,You Zhou,Yuanji Ma,Juying Qian,Junbo Ge

Frontiers in immunology 14:1170388 PubMed37122744

2023

Nrf2 orchestrates transition from acute to chronic otitis media through inflammatory macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Fan,Hongming Xu,Chenling Shen,Jia Fang,Xiaoyan Li

Frontiers in immunology 14:1165602 PubMed37077909

2023

Loss of NLRP3 reduces oxidative stress and polarizes intratumor macrophages to attenuate immune attack on endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Zhu,Yanli Xu,Juan Wang,Zhuowei Xue,Tian Qiu,Jing Chen

Frontiers in immunology 13:1064303 PubMed36505450

2022

Persistent expression of NLRP3 in spinal microglia promotes development of lumbar disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Jing Zhang

Frontiers in immunology 13:1057746 PubMed36405726

2022

NLRP3+ macrophages aggravate inflammatory cystitis in diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Yubing Peng,Yan Gao

Disease markers 2022:9883831 PubMed36133436

2022

Activated Hepatic Stellate Cells Promote the M1 to M2 Macrophage Transformation and Liver Fibrosis by Elevating the Histone Acetylation Level.

Applications

Unspecified application

Species

Unspecified reactive species

Junru Chen,Xueqing Huang,Zhi Huang,Yu Cao
View all publications
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