AB206984

Mouse CD23 ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Mouse CD23 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CD23 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD23, Fcer2a, Fcer2, Low affinity immunoglobulin epsilon Fc receptor, Fc-epsilon-RII, Lymphocyte IgE receptor

3 Images

Sandwich ELISA - Mouse CD23 ELISA Kit (AB206984)

sELISA

Supplier Data

Sandwich ELISA - Mouse CD23 ELISA Kit (AB206984)

Example of CD23 standard curve in Sample Diluent 25BS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse CD23 ELISA Kit (AB206984)

Interpolated concentrations of CD23 in mouse serum, plasma and cell culture supernatant samples.

The concentrations of CD23 were measured in duplicates, interpolated from the CD23 standard curves and corrected for sample dilution. Note that 1X Diluted serum samples were pre-diluted to 2.5%. Note that 1X Diluted plasma samples were pre-diluted to 5%. Note that 1X Diluted Mouse spleen Day 5 cell culture supernatant samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are graphed in pg of CD23 per mL of neat sample (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse CD23 ELISA Kit (AB206984)

Example of CD23 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse CD23 ELISA Kit (ab206984) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD23 protein in cell culture supernatant, serum, cit plasma, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD23 with 2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD23 is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. It has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). It is a 47 kDa type II transmembrane glycoprotein containing a short N-terminal cytoplasmic tail, a transmembrane domain, and extracellular domain containing C-type lectin domain. Two membrane isoforms and soluble CD23 fragements exist.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" } ]

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Recovery

[ { "sample": "Serum", "range": "119.2 - 133.4 %", "average": "= 126.1" }, { "sample": "EDTA Plasma", "range": "85.8 - 92.3 %", "average": "= 89.2" }, { "sample": "Citrate plasma", "range": "77.8 - 83.9 %", "average": "= 81.3" }, { "sample": "Cell culture media", "range": "117 - 123.3 %", "average": "= 120.7" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab206984-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB206984-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB206984-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD23 Capture Antibody", "number":"AB206984-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CD23 Lyophilized Recombinant Protein", "number":"AB206984-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB206984-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB206984-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB206984-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB206984-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD23 Detector Antibody", "number":"AB206984-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB206984-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB206984-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD23 also known as Fc epsilon receptor II (FcεRII) or BU38 is a type II integral membrane protein with a molecular mass of approximately 45 kDa. It is expressed mainly on B cells but also appears on various other cell types such as macrophages eosinophils and Langerhans cells. CD23 expression occurs in both soluble and membrane-bound forms. The protein consists of a lectin-like extracellular domain a short transmembrane domain and a cytoplasmic tail.

Biological function summary

CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.

Pathways

CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.

CD23 is most notably associated with allergic diseases such as asthma and allergic rhinitis due to its role in IgE regulation. It is also linked to chronic lymphocytic leukemia (CLL) in which CD23 serves as a marker for disease progression. In CLL interactions involving CD23 and related proteins such as BCMA and BAFF have been studied to better understand the disorder and its treatment. Anti-CD23 antibodies have been used as both diagnostic tools and therapeutic agents to target CD23-positive cells in these diseases.

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Product protocols

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Target data

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway.

See full target information Fcer2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular immunology 381:104603 PubMed36182705

2022

BAFF antagonism via the BAFF receptor 3 binding site attenuates BAFF 60-mer-induced classical NF-κB signaling and metabolic reprogramming of B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa D Lempicki,Saikat Paul,Vlad Serbulea,Clint M Upchurch,Srabani Sahu,Jake A Gray,Gorav Ailawadi,Brandon L Garcia,Coleen A McNamara,Norbert Leitinger,Akshaya K Meher

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse CD23 ELISA Kit

Sandwich ELISA - Mouse CD23 ELISA Kit (AB206984)

Sandwich ELISA - Mouse CD23 ELISA Kit (AB206984)

Sandwich ELISA - Mouse CD23 ELISA Kit (AB206984)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise