JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB233619

Mouse CD27 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse CD27 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CD27 with a sensitivity of 10.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD27, Tnfrsf7, Cd27, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7

3 Images
Sandwich ELISA - Mouse CD27 ELISA Kit (AB233619)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD27 ELISA Kit (AB233619)

Interpolated concentrations of native CD27 in mouse EL4-IL2 cell culture supernatant samples, both untreated and treated with 10 µg/ml PHA and 10 ng/ml PMA for two days.

The concentrations of CD27 were measured in duplicates, interpolated from the CD27 standard curves and corrected for sample dilution. Undiluted samples are as follows : untreated EL4-IL2 supernatant 100% and treated EL4-IL2 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD27 concentration was determined to be 426 pg/mL in untreated EL4-IL2 supernatant and 1,978 pg/ml in treated EL4-IL2 supernatant.

Sandwich ELISA - Mouse CD27 ELISA Kit (AB233619)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD27 ELISA Kit (AB233619)

Interpolated concentrations of native CD27 in mouse serum and plasma.

The concentrations of CD27 were measured in duplicates, interpolated from the CD27 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, plasma (heparin) 12.5%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD27 concentration was determined to be 14.3 ng/mL in serum, 11.9 ng/mL in plasma (citrate), 13.4 g/ml in plasma (heparin), and 10.8 ng/ml in plasma (EDTA).

Sandwich ELISA - Mouse CD27 ELISA Kit (AB233619)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD27 ELISA Kit (AB233619)

Example of mouse CD27 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.9 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB236155

Anti-CD27 antibody [EPR20585] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (AB236155)

  • 0
  • 0
Assay Kits

AB233620

Mouse CD93 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)

  • 0
  • 0
Assay Kits

AB208348

Mouse TNF alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

  • 4
  • 4
Proteins & Peptides

AB316470

Recombinant Human CD27 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CD27 Protein Standard (His tag) (AB316470)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse CD27 ELISA Kit (ab233619) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD27 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD27 with 10.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 108 %", "average": "= 104" }, { "sample": "Serum", "range": "93 - 106 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "79 - 99 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "99 - 109 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "88 - 98 %", "average": "= 94" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233619-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233619-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233619-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233619-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233619-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233619-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233619-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD27 Capture Antibody", "number":"AB233619-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB233619-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CD27 Lyophilized Recombinant Protein", "number":"AB233619-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD27 Detector Antibody", "number":"AB233619-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.
Biological function summary

One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.

Pathways

CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.

CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.
style
left-column

Product protocols

style
left-column

Target data

Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed : 11062504). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (PubMed : 15723067). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (PubMed : 38386557).
See full target information Cd27
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com