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AB113341

Mouse CD30 ELISA Kit

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Mouse CD30 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse CD30 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD30, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8

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Sandwich ELISA - Mouse CD30 ELISA Kit (AB113341)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD30 ELISA Kit (AB113341)

Representative Standard Curve using ab113341

Sandwich ELISA - Mouse CD30 ELISA Kit (AB113341)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD30 ELISA Kit (AB113341)

Representative Standard Curve using ab113341

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

12.29 - 3000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CD30 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD30 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse CD30 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD30 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD30 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD30 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "69 - 87 %", "average": "= 77.29" }, { "sample": "Serum", "range": "68 - 84 %", "average": "= 75.99" }, { "sample": "Plasma", "range": "67 - 83 %", "average": "= 74.88" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113341-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113341-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113341-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD30 Microplate (12 strips x 8 wells)", "number":"AB113341-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse CD30", "number":"AB113341-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB113341-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB113341-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113341-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse CD30 Standard (lyophilized)", "number":"AB113341-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113341-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Receptor for TNFSF8/CD30L (PubMed : 8543792). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (By similarity).
See full target information Tnfrsf8
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com