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AB264614

Mouse CD30 ELISA Kit

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Mouse CD30 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CD30 with a sensitivity of 79 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD30, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8

2 Images
Sandwich ELISA - Mouse CD30 ELISA Kit (AB264614)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD30 ELISA Kit (AB264614)

Interpolated concentrations of native CD30 in mouse cell culture supernatant samples.

The concentrations of CD30 were measured in duplicates, interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are as follows : Stimulated EL4 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 5.3 ng/mL in stimulated EL4 supernatant.

Sandwich ELISA - Mouse CD30 ELISA Kit (AB264614)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD30 ELISA Kit (AB264614)

Example of mouse CD30 standard curve in Sample Diluent NS.

The CD30 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 79 pg/mL

Range

133 - 8500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CD30 ELISA Kit (ab264614) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD30 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD30 with 79 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD30, also known as Tumor necrosis factor receptor superfamily member 8, is a cell membrane receptor encoded by the gene Tnfrsf8. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 119 %", "average": "= 106" }, { "sample": "Serum", "range": "98 - 114 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "90 - 106 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "93 - 108 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "87 - 102 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264614-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264614-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264614-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264614-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264614-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264614-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264614-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CD30 Lyophilized Recombinant Protein", "number":"AB264614-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB264614-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD30 Capture Antibody", "number":"AB264614-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10x Mouse CD30 Detector Antibody", "number":"AB264614-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for TNFSF8/CD30L (PubMed : 8543792). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (By similarity).
See full target information Tnfrsf8
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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