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AB100674

Mouse CD40 ELISA Kit

4

(2 Reviews)

|

(4 Publications)

Mouse CD40 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse CD40 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor

2 Images
Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)

Representative Standard Curve using ab100674

Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)

Representative Standard Curve using ab100674

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 3 pg/mL

Range

2.06 - 1500 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CD40 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse CD40 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Mouse CD40 ELISA Kit (TNFRS5) (ab206307) from our SimpleStep ELISA® range.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "74 - 93 %", "average": "= 84.23" }, { "sample": "Serum", "range": "71 - 87 %", "average": "= 77.26" }, { "sample": "Plasma", "range": "69 - 87 %", "average": "= 76.68" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100674-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100674-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100674-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD40 Microplate (12 x 8 wells)", "number":"AB100674-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse CD40", "number":"AB100674-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse CD40 Standard (lyophilized)", "number":"AB100674-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100674-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100674-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100674-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100674-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed : 12881420).
See full target information Cd40
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:17262 PubMed34446808

2021

Downregulation of CD40L-CD40 attenuates seizure susceptibility and severity of seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Pototskiy,Katherine Vinokuroff,Andrew Ojeda,C Kendall Major,Deepak Sharma,Taylor Anderson,Kendall Howard,Ronen Borenstein,Alberto E Musto

Experimental and therapeutic medicine 16:3261-3267 PubMed30233673

2018

Regulatory role of microRNA-185 in the recovery process after ankle fracture.

Applications

Unspecified application

Species

Unspecified reactive species

Deping Sun,Juntao Liu,Qingpeng Shi,Haibo Mu,Dongsheng Zhou

Experimental and therapeutic medicine 15:4550-4556 PubMed29849780

2018

Aberrant expression of miR-663 and transforming growth factor-β1 in nasal polyposis in children.

Applications

Unspecified application

Species

Unspecified reactive species

Hailing Yu,Jianbao Ju,Jingdong Liu,Da Li

Experimental and therapeutic medicine 13:1127-1132 PubMed28450952

2017

MicroRNA-185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor-β1.

Applications

ELISA

Species

Human

Pengfei Zhao,Shaochun Wang,Yingjie Zhou,Huailiang Zheng,Gang Zhao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com