Mouse CD40 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse CD40 in Mouse in Plasma, Cell culture supernatant, Serum samples.
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- Scientific reports 11:172622021Downregulation of CD40L-CD40 attenuates seizure susceptibility and severity of seizures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEsther Pototskiy,Katherine Vinokuroff,Andrew Ojeda,C Kendall Major,Deepak Sharma,Taylor Anderson,Kendall Howard,Ronen Borenstein,Alberto E MustoPubMed 34446808
- Experimental and therapeutic medicine 16:3261-32672018Regulatory role of microRNA-185 in the recovery process after ankle fracture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeping Sun et. al.PubMed 30233673
- Experimental and therapeutic medicine 15:4550-45562018Aberrant expression of miR-663 and transforming growth factor-β1 in nasal polyposis in children.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHailing Yu et. al.PubMed 29849780
- Experimental and therapeutic medicine 13:1127-11322017MicroRNA-185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor-β1.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 2.06 - 1500 pg/mL
- Sensitivity
- < 3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).
Alternative names
CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor
What's included?
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Mouse CD40 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse CD40 in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 2.06 - 1500 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 3 pg/mL
- General recovery
- 86%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 84.23 | Range 74 - 93 % |
Sample type Serum | Average % = 77.26 | Range 71 - 87 % |
Sample type Plasma | Average % = 76.68 | Range 69 - 87 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's CD40 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CD40 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Mouse CD40 ELISA Kit (TNFRS5) (ab206307) from our SimpleStep ELISA® range.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Mouse CD40 ELISA Kit (ab100674)
Representative Standard Curve using ab100674
Sandwich ELISA - Mouse CD40 ELISA Kit (ab100674)
Representative Standard Curve using ab100674
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Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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