AB119517

Mouse CD40L ELISA Kit

Name: Mouse CD40L ELISA Kit (ab119517) | Abcam
Brand: Abcam Limited
SKU: AB119517
Price: 780 USD
Availability: InStock

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(4 Publications)

Mouse CD40L ELISA Kit is a Sandwich ELISA for the measurement of Mouse CD40L in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

CD154, Cd40l, Tnfsf5, Cd40lg, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, TRAP

1 Images

Sandwich ELISA - Mouse CD40L ELISA Kit (AB119517)

sELISA

Supplier Data

Sandwich ELISA - Mouse CD40L ELISA Kit (AB119517)

Representative Standard Curve using ab119517.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.14 ng/mL

Range

0.31 - 20 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's CD40L Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse CD40L concentrations in cell culture supernatant and serum.

CD40L specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated CD40L detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD40L captured on the plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119517-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB119517-CMP10", "productcode":"" }, { "size":"1 x 5 mL", "name":"20X Assay Buffer Concentrate", "number":"AB119517-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent", "number":"AB119517-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate coated with monoclonal antibody to mouse CD40L (12 x 8 wells)", "number":"AB119517-CMP06", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Films", "number":"AB119517-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CD40L Standard lyophilized (40 ng /mL upon reconstitution)", "number":"AB119517-CMP04", "productcode":"" }, { "size":"1 x 70 µL", "name":"Biotin-Conjugate anti-mouse CD40L monoclonal antibody", "number":"AB119517-CMP03", "productcode":"" }, { "size":"1 x 150 µL", "name":"Streptavidin-HRP", "number":"AB119517-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB119517-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution (1M Phosphoric acid)", "number":"AB119517-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.

Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.

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Product protocols

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Target data

Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed : 1374165). Involved in immunoglobulin class switching (PubMed : 1374165).. CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5 : ITGB1 and ITGAV : ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

See full target information Cd40lg

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35457070

2022

The Memory Benefit to Aged APP/PS1 Mice from Long-Term Intranasal Treatment of Low-Dose THC.

Applications

Unspecified application

Species

Unspecified reactive species

Oksana Fihurka,Yuzhu Hong,Jiyu Yan,Breanna Brown,Xiaoyang Lin,Ning Shen,Yanhong Wang,Haohan Zhao,Marcia N Gordon,David Morgan,Qingyu Zhou,Ping Chang,Chuanhai Cao

Scientific reports 11:17262 PubMed34446808

2021

Downregulation of CD40L-CD40 attenuates seizure susceptibility and severity of seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Pototskiy,Katherine Vinokuroff,Andrew Ojeda,C Kendall Major,Deepak Sharma,Taylor Anderson,Kendall Howard,Ronen Borenstein,Alberto E Musto

Nature communications 12:3754 PubMed34145241

2021

Cell-specific and divergent roles of the CD40L-CD40 axis in atherosclerotic vascular disease.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Lacy,Christina Bürger,Annelie Shami,Maiwand Ahmadsei,Holger Winkels,Katrin Nitz,Claudia M van Tiel,Tom T P Seijkens,Pascal J H Kusters,Ela Karshovka,Koen H M Prange,Yuting Wu,Sanne L N Brouns,Sigrid Unterlugauer,Marijke J E Kuijpers,Myrthe E Reiche,Sabine Steffens,Andreas Edsfeldt,Remco T A Megens,Johan W M Heemskerk,Isabel Goncalves,Christian Weber,Norbert Gerdes,Dorothee Atzler,Esther Lutgens

Journal for immunotherapy of cancer 7:214 PubMed31399043

2019

Development of a new fusion-enhanced oncolytic immunotherapy platform based on herpes simplex virus type 1.

Applications

Unspecified application

Species

Unspecified reactive species

Suzanne Thomas,Linta Kuncheria,Victoria Roulstone,Joan N Kyula,David Mansfield,Praveen K Bommareddy,Henry Smith,Howard L Kaufman,Kevin J Harrington,Robert S Coffin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse CD40L ELISA Kit

Sandwich ELISA - Mouse CD40L ELISA Kit (AB119517)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise