AB213851

Mouse CD56 ELISA Kit (NCAM1)

Name: Mouse CD56 ELISA Kit (NCAM1) (ab213851) | Abcam
Brand: Abcam Limited
SKU: AB213851
Price: 780 USD
Availability: InStock

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Mouse CD56 ELISA Kit (NCAM1) is a Sandwich (quantitative) ELISA for the measurement of Mouse CD56 (NCAM1) in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

CD56, Ncam, Ncam1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

1 Images

Sandwich ELISA - Mouse CD56 ELISA Kit (NCAM1) (AB213851)

sELISA

Supplier Data

Sandwich ELISA - Mouse CD56 ELISA Kit (NCAM1) (AB213851)

Mouse CD56 ELISA Kit (NCAM1) (ab213851) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

625 - 40000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

The Mouse CD56 Enzyme-Linked Immunosorbent Assay (ELISA) kit (NCAM1) (ab213851) is designed for the quantitative measurement of Mouse CD56 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD56/NCAM1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: L20-T711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56/NCAM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CD56/NCAM1 amount of sample captured in plate.

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of the Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms. The NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "4.632 ng/mL", "standardDeviation": "0.342", "coefficientOfVariability": "= 7.4" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "4.645 pg/mL", "standardDeviation": "0.422", "coefficientOfVariability": "= 9.1" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "16.195 pg/mL", "standardDeviation": "1.214", "coefficientOfVariability": "= 7.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "15.378 ng/mL", "standardDeviation": "0.953", "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1.401 pg/mL", "standardDeviation": "0.12", "coefficientOfVariability": "= 8.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1.344 ng/mL", "standardDeviation": "0.09", "coefficientOfVariability": "= 6.7" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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Product protocols

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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

See full target information Ncam1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse CD56 ELISA Kit (NCAM1)

Sandwich ELISA - Mouse CD56 ELISA Kit (NCAM1) (AB213851)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise