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AB213852

Mouse CD5L / CT-2 ELISA Kit

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Mouse CD5L / CT-2 ELISA Kit is a Sandwich ELISA for the measurement of Mouse CD5L / CT-2 in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Aim, Api6, Cd5l, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, Apoptosis inhibitory 6, SP-alpha, mAIM

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Sandwich ELISA - Mouse CD5L / CT-2 ELISA Kit (AB213852)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD5L / CT-2 ELISA Kit (AB213852)

Mouse CD5L / CT-2 ELISA Kit (ab213852) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse CD5L / CT-2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213852) is designed for the quantitative measurement of activated Mouse CD5L / CT-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CD5L / CT-2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E22-V352) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5L / CT-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CD5L / CT-2 amount of sample captured in plate.

CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr/Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "207 pg/mL", "standardDeviation": "17.38", "coefficientOfVariability": "= 8.4" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1789 pg/mL", "standardDeviation": "110.91", "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4940 pg/mL", "standardDeviation": "375.44", "coefficientOfVariability": "= 7.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "201 pg/mL", "standardDeviation": "13.26", "coefficientOfVariability": "= 6.6" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1935 pg/mL", "standardDeviation": "131.58", "coefficientOfVariability": "= 6.8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4821 pg/mL", "standardDeviation": "414.6", "coefficientOfVariability": "= 8.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD5L also known as CT-2 or CD5 antigen-like is a secreted glycoprotein with a molecular mass of approximately 50 kDa. This protein expresses mainly in liver and to a lesser extent in spleen lymph nodes and other immune-related tissues. The CD5L protein acts as a scavenger receptor cysteine-rich (SRCR) superfamily member which participates in pathogen recognition and clearance. Researchers often measure CD5L levels using CD5L ELISA kits providing quantitative analysis of this biomarker in various biological samples.
Biological function summary

CD5L influences several immune processes modulating macrophage function and lipid metabolism. It plays a role within a complex system transforming macrophages into an anti-inflammatory phenotype. This helps the clearance of apoptotic cells and prevents tissue damage during inflammation. CD5L also impacts cholesterol homeostasis contributing to lipid clearance and influencing foam cell formation which serves a significant role in atherosclerosis development.

Pathways

This protein integrates into the lipid metabolism pathway and the immune regulation pathway. Within the lipid metabolism pathway interaction with proteins such as apolipoprotein AI demonstrates its role in controlling cholesterol and triglyceride levels. In the immune regulation pathway CD5L collaborates with other immune-related proteins including CD36 and TLR2 to regulate immune response and inflammation affecting macrophage activation and phagocytosis.

CD5L associates with atherosclerosis and systemic lupus erythematosus (SLE). Researchers link elevated CD5L levels with atherosclerosis due to its ability to drive macrophage transformation into foam cells linking it with cardiovascular diseases. In systemic lupus erythematosus CD5L interacts with protein serum amyloid A (SAA) exacerbating the inflammatory processes associated with autoimmune disorders. Understanding CD5L's role may provide insights for developing therapeutic strategies for these conditions.
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Product protocols

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Target data

Secreted protein that acts as a key regulator of lipid synthesis : mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis (PubMed : 26048980). Able to inhibit lipid droplet size in adipocytes (PubMed : 20519120, PubMed : 22579686). Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA) (PubMed : 20519120). CD5L-induced lipolysis occurs with progression of obesity : participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease (PubMed : 21730133). Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC) (PubMed : 22579686, PubMed : 26607793). Acts as a key regulator of metabolic switch in T-helper Th17 cells (PubMed : 26607793, PubMed : 26607794). Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation (PubMed : 26607793). CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes (PubMed : 26607793). Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies (PubMed : 23562157). Also acts as an inhibitor of apoptosis in macrophages : promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed : 16054063, PubMed : 9892623). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (By similarity). Promotes recovery from acute kidney injury (AKI) by accumulating on necrotic cell debris within the kidney proximal tubules following AKI and interacting with HAVCR1 expressed on the surface of injured kidney tubular epithelial cells (PubMed : 26726878). This enhances HAVCR1-mediated phagocytosis of intraluminal necrotic debris and contributes to kidney tissue repair (PubMed : 26726878). Also enhances necrotic debris uptake by kidney macrophages in a HAVCR1-independent manner (PubMed : 26726878).
See full target information Cd5l
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