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AB245721

Mouse CD80 ELISA Kit

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Mouse CD80 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CD80 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

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Sandwich ELISA - Mouse CD80 ELISA Kit (AB245721)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD80 ELISA Kit (AB245721)

Example of mouse CD80 standard curve in 1X Cell Extraction Buffer PTR. The CD80 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CD80 ELISA Kit (AB245721)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD80 ELISA Kit (AB245721)

Interpolated concentrations of native CD80 in mouse C2C12 cell extract based on a 10 μg/mL extract load. The concentrations of CD80 were measured in duplicate and interpolated from the CD80 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 314.7 pg/mL in C2C12 cell extract.

Sandwich ELISA - Mouse CD80 ELISA Kit (AB245721)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD80 ELISA Kit (AB245721)

Interpolated concentrations of native CD80 in mouse serum, plasma, and cell culture supernatant samples. The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (heparin) 25%, and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 1,505.1 pg/mL in serum, 829.0 pg/mL in plasma (citrate), 1,487.5 pg/mL in plasma (heparin), and 228.8 pg/ml in spleen supernatant.

Sandwich ELISA - Mouse CD80 ELISA Kit (AB245721)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD80 ELISA Kit (AB245721)

Example of mouse CD80 standard curve in Sample Diluent NS. The CD80 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CD80 ELISA Kit (ab245721) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD80 protein in cell culture extracts, cell culture supernatant, cit plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD80 with 2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 113 %", "average": "= 106" }, { "sample": "Serum", "range": "91 - 110 %", "average": "= 101" }, { "sample": "Cell culture extracts", "range": "88 - 106 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "108 - 115 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "99 - 117 %", "average": "= 107" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245721-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245721-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB245721-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245721-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD80 Detector Antibody", "number":"AB245721-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245721-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB245721-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245721-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245721-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245721-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD80 Capture Antibody", "number":"AB245721-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CD80 Lyophilized recombinant Protein", "number":"AB245721-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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Product protocols

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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed : 22450810).
See full target information Cd80
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Product promise

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