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AB233620

Mouse CD93 ELISA Kit

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Mouse CD93 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CD93 with a sensitivity of 3.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD93, Aa4, C1qr1, C1qrp, Ly68, Cd93, Complement component C1q receptor, C1q/MBL/SPA receptor, Cell surface antigen AA4, Complement component 1 q subcomponent receptor 1, Lymphocyte antigen 68, C1qR(p), C1qRp, Ly-68

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Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)

Example of mouse CD93 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)

Mouse CD93 Competitor standard curve comparison.

Standard curve comparison between Mouse CD93 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)

Interpolated concentrations of native CD93 in mouse tissue culture supernatant samples.

The concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse lung supernatant 1 : 200 and mouse heart supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 103 ng/mL in mouse lung supernatant and 50 ng/ml in mouse heart supernatant.

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)

Example of mouse CD93 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)

Interpolated concentrations of native CD93 in mouse lung, heart, and spleen homogenate extract samples based on a 3 μg/mL, 25 μg/mL, and 50 μg/mL extract load respectively.

The concentrations of CD93 were measured in duplicate and interpolated from the CD93 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 898 pg/mL in lung homogenate extract, 1116 pg/ml in heart homogenate extract, and 1133 ng/mL in spleen homogenate extract samples.

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CD93 ELISA Kit (AB233620)

Interpolated concentrations of native CD93 in mouse serum and plasma samples.

The concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1,000, plasma (citrate) 1 : 500, plasma (heparin) 1 : 1,000, and plasma (EDTA) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 863 ng/mL in serum, 633 ng/mL in plasma (citrate), 799 ng/ml in plasma (heparin), and 468 ng/ml in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Tissue Homogenate, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.4 pg/mL

Range

23.4 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CD93 ELISA Kit (ab233620) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD93 protein in edta plasma, hep plasma, serum, tissue extracts, tissue homogenate, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD93 with 3.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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Recovery

[ { "sample": "Serum", "range": "98 - 107 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "98 - 106 %", "average": "= 101" }, { "sample": "Tissue Extracts", "range": "97 - 112 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "101 - 113 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "99 - 110 %", "average": "= 103" }, { "sample": "Tissue Homogenate", "range": "102 - 117 %", "average": "= 108" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233620-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233620-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233620-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233620-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB233620-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233620-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233620-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233620-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB233620-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CD93 Lyophilized Recombinant Protein", "number":"AB233620-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD93 Capture Antibody", "number":"AB233620-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB233620-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CD93 Detector Antibody", "number":"AB233620-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD93 also known as C1q receptor and a type I transmembrane glycoprotein weighs approximately 68 kDa. This protein is mainly expressed on platelets monocytes endothelial cells and some cell lines within the immune system. CD93 is known for engaging in cell adhesion and phagocytosis processes. It has a notable presence on the surface of circulating cells indicating its involvement in physiological interactions essential for cellular communication.
Biological function summary

CD93 contributes to cellular processes through its role in the clearance of apoptotic cells and immune surveillance. It also partakes in angiogenesis facilitating the growth of new blood vessels. CD93 often functions as part of a larger complex in binding with other molecules to support cellular communication. By modulating the immune response CD93 ensures efficient removal of cellular debris maintaining homeostasis and preventing autoimmune reactions.

Pathways

CD93 operates within the innate immune response and complement system pathways. It closely interacts with other proteins such as C1q which is part of the complement activation pathway. This interaction supports the clearance of immune complexes and cellular debris. By facilitating these processes CD93 aids in maintaining the balance between immune activation and tolerance.

CD93 has associations with inflammatory diseases and cancer progression. In inflammatory diseases its role in the immune system implicates it in conditions characterized by excessive inflammation often relating to the misregulation of immune functions. As for cancer CD93's involvement in angiogenesis links it to tumor growth with connections seen alongside vascular endothelial growth factor (VEGF) pathways. These insights into CD93's role suggest its potential as a therapeutic target in treating related pathological states.
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Product protocols

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Target data

Cell surface receptor that plays a role in various physiological processes including inflammation, phagocytosis, and cell adhesion (PubMed : 15004139, PubMed : 20439917, PubMed : 38441970). Plays a role in phagocytosis and enhances the uptake of apoptotic cells and immune complexes by acting as a receptor for defense collagens including surfactant protein A/SFTPA1, C1q, and mannose-binding lectin (MBL2) (PubMed : 15004139). Plays a role in the regulation of endothelial cell function and adhesion by activating angiogenesis. Mechanistically, exerts its angiogenic function by associating with beta-dystroglycan, leading to SRC-dependent phosphorylation and subsequent recruitment of CBL. In turn, CBL provides a docking site for downstream signaling components, such as CRKL to enhance cell migration. Participates in angiogenesis also by acting as a receptor for the ECM pan-endothelial glycoprotein multimerin-2/MMRN2 and IGFBP7 ligands. Both ligands play a non-redundant role in CD93-mediated endothelial cell function (By similarity). Acts as a key regulator of endothelial barrier function through modulating VEGFR2 function (PubMed : 38441970).
See full target information Cd93
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websiteProtocolBooklet
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Product promise

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