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AB230131

Mouse CDKN2A/p16INK4a ELISA Kit

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(2 Publications)

Mouse CDKN2A/p16INK4a ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CDKN2A/p16INK4a with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Tumor suppressor ARF, Alternative reading frame, Cyclin-dependent kinase inhibitor 2A, p19ARF, ARF, Cdkn2a

4 Images
ELISA - Mouse CDKN2A/p16INK4a ELISA Kit (AB230131)
  • ELISA

Supplier Data

ELISA - Mouse CDKN2A/p16INK4a ELISA Kit (AB230131)
Sandwich ELISA - Mouse CDKN2A/p16INK4a ELISA Kit (AB230131)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CDKN2A/p16INK4a ELISA Kit (AB230131)

Representative image of Mouse CDKN2A/p16INK4a ELISA Kit (ab230131)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse CDKN2A/p16INK4a Capture Antibody

- 10X Mouse CDKN2A/p16INK4a Detector Antibody

- Mouse CDKN2A/p16INK4a Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Mouse CDKN2A/p16INK4a ELISA Kit (AB230131)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CDKN2A/p16INK4a ELISA Kit (AB230131)

Interpolated concentrations of native CDKN2A in Mouse MEF extract based on a 20 μg/mL extract load.

The concentrations of CDKN2A/p16INK4a were measured in duplicate and interpolated from the CDKN2A/p16INK4a standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The average interpolated amount of native CDKN2A/p16INK4a is 548 pg/ml in MEF extract.

Sandwich ELISA - Mouse CDKN2A/p16INK4a ELISA Kit (AB230131)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CDKN2A/p16INK4a ELISA Kit (AB230131)

Example of Mouse CDKN2A standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8 pg/mL

Range

22 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CDKN2A/p16INK4a ELISA Kit ab230131 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse CDKN2A/p16INK4a in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 8 pg/mL.

How the assay works

Mouse CDKN2A/p16INK4a SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse CDKN2A/p16INK4a ELISA Kit ab230131 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CDKN2A/p16INK4a antibody [EPR20418-129] - BSA and Azide free (Capture) ab244969
- Anti-CDKN2A/p16INK4a antibody [EPR20418-167] - BSA and Azide free (Detector) ab244969

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "MEF Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "MEF Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "110 - 119 %", "average": "= 116" }, { "sample": "Tissue Extracts", "range": "86 - 95 %", "average": "= 90" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab230131-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB230131-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB230131-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB230131-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB230131-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB230131-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB230131-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB230131-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB230131-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CDKN2A Detector Antibody", "number":"AB230131-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB230131-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CDKN2A Lyophilized Recombinant Protein", "number":"AB230131-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CDKN2A Capture Antibody", "number":"AB230131-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Capable of inducing cell cycle arrest in G1 and G2 phases (PubMed : 8521522, PubMed : 9393858). Acts as a tumor suppressor (PubMed : 15601844, PubMed : 17936562, PubMed : 8521522, PubMed : 9393858). Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus (PubMed : 10359817, PubMed : 9529248). This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis (PubMed : 10359817). Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes (PubMed : 15361884). Binds to BCL6 and down-regulates BCL6-induced transcriptional repression (PubMed : 15567177). Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression (By similarity). Binds to TOP1/TOPOI and stimulates its activity (By similarity). This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation (By similarity). Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing (By similarity). Plays a role in inhibiting ribosome biogenesis, perhaps by binding to the nucleolar localization sequence of transcription termination factor TTF1, and thereby preventing nucleolar localization of TTF1 (PubMed : 20513429). Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination (By similarity). Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1 (By similarity). Binds to HUWE1 and represses its ubiquitin ligase activity (By similarity). May play a role in controlling cell proliferation and apoptosis during mammary gland development (By similarity).. Isoform smARF. May be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.
See full target information Cdkn2a
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7: PubMed33536212

2021

High-resolution 3D imaging uncovers organ-specific vascular control of tissue aging.

Applications

Unspecified application

Species

Unspecified reactive species

Junyu Chen,Unnikrishnan Sivan,Sin Lih Tan,Luciana Lippo,Jessica De Angelis,Rossella Labella,Amit Singh,Alexandros Chatzis,Stanley Cheuk,Mino Medhghalchi,Jesus Gil,Georg Hollander,Brian D Marsden,Richard Williams,Saravana K Ramasamy,Anjali P Kusumbe

Communications biology 2:307 PubMed31428695

2019

Targeting p16-induced senescence prevents cigarette smoke-induced emphysema by promoting IGF1/Akt1 signaling in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher T Cottage,Norman Peterson,Jennifer Kearley,Aaron Berlin,Ximing Xiong,Anna Huntley,Weiguang Zhao,Charles Brown,Annik Migneault,Kamelia Zerrouki,Gerald Criner,Roland Kolbeck,Jane Connor,Raphael Lemaire
View all publications
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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