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AB282305

Mouse CDNF ELISA Kit

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Mouse CDNF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CDNF with a sensitivity of 1.026 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Armetl1, Cdnf, Cerebral dopamine neurotrophic factor, ARMET-like protein 1, Conserved dopamine neurotrophic factor

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Sandwich ELISA - Mouse CDNF ELISA Kit (AB282305)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CDNF ELISA Kit (AB282305)

Example of mouse CDNF standard curve in Sample Diluent NS.

The CDNF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CDNF ELISA Kit (AB282305)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CDNF ELISA Kit (AB282305)

Interpolated concentrations of native CDNF in mouse brain tissue based on a 1,000 μg/mL extract load and neuro2a cell extract based on a 250 μg/mL extract load.

The concentrations of CDNF were measured in duplicate and interpolated from the CDNF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CDNF concentration was determined to be 32.85 pg/mL in brain tissue extract and 87.94 pg/mL in neuro2a cell extract.

Sandwich ELISA - Mouse CDNF ELISA Kit (AB282305)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CDNF ELISA Kit (AB282305)

Interpolated concentrations of native CDNF in mouse serum, plasma and cell culture supernatant samples.

The concentrations of CDNF were measured in duplicates, interpolated from the CDNF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CDNF concentration was determined to be 55.68 pg/mL in serum, 46.13 pg/mL in plasma (citrate), 43.91 pg/mL in plasma (EDTA), 46.26 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse CDNF ELISA Kit (AB282305)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CDNF ELISA Kit (AB282305)

Representative image of Mouse CDNF ELISA Kit (ab282305)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse CDNF Capture Antibody

- 10X Mouse CDNF Detector Antibody

- Mouse CDNF Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.026 pg/mL

Range

2.344 - 150 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CDNF ELISA kit (ab282305) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse CDNF protein in mouse serum, plasma, cell culture supernatant and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CDNF with 1.41 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse Cerebral dopamine neurotrophic factor (CDNF), a trophic factor for dopamine neurons, prevents degeneration induced by 6-hydroxydopamine (6-OHDA) of dopaminergic neurons. CDNF restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (by similarity) when administered after 6-OHDA-lesioning.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]
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Recovery

[ { "sample": "Serum", "range": "81 - 103 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "80 - 99 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "92 - 99 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "81 - 92 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "81 - 104 %", "average": "= 93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282305-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282305-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282305-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CDNF Detector Antibody", "number":"AB282305-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282305-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB282305-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB282305-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282305-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282305-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282305-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB282305-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CDNF Lyophilized Recombinant Protein", "number":"AB282305-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CDNF Capture Antibody", "number":"AB282305-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDNF also known as Cerebral Dopamine Neurotrophic Factor is a protein that helps in maintaining the proper function of neurons. It has a mass of approximately 18 kDa. This protein is widely expressed in various tissues especially in the brain. CDNF maintains endoplasmic reticulum homeostasis and is involved in the stabilization of dopaminergic neurons. It does this by reducing stress in the endoplasmic reticulum and promoting cell survival mechanisms.
Biological function summary

The protein ensures the survival of neurons by modulating cellular stress pathways. CDNF is not known to be part of a larger protein complex. Instead its activity relies on its own structural configuration to influence stress responses and neuron protection. As a secreted protein it directly interacts with and influences cellular mechanisms within the extracellular space contributing to a healthy neuronal environment.

Pathways

CDNF interacts with at least oxidative stress response pathways and neuroprotection pathways. These pathways underpin its role in counteracting cellular stress related to neurodegeneration. For example CDNF shares a functional connection with proteins like MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) another neurotrophic factor involved in similar neuroprotective pathways enhancing neuronal survival and recovery from cellular stress.

Neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease are major areas where CDNF shows relevance. In Parkinson's disease CDNF's neuroprotective role helps mitigate the loss of dopaminergic neurons. There is evidence linking CDNF with MANF which also exhibits protective roles in neuronal health. Both proteins aim to promote neuron survival amid progression of neurodegeneration associated with these diseases indicating their potential as therapeutic agents.
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Product protocols

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Target data

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).
See full target information Cdnf
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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