Mouse Chemerin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Chemerin with a sensitivity of 1.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Breast cancer research : BCR 22:1032020Sunitinib facilitates metastatic breast cancer spreading by inducing endothelial cell senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenian Wang et. al.PubMed 32993785
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 14.06 - 900 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.75 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Acts also as a ligand for CMKLR2. Can also bind to C-C chemokine receptor-like 2 (CCRL2), but with a lower affinity than it does to CMKLR1 or CMKLR2. Positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Also acts as a pro-inflammatory adipokine, causing an increase in secretion of pro-inflammatory and prodiabetic adipokines, which further impair adipose tissue metabolic function and have negative systemic effects including impaired insulin sensitivity, altered glucose and lipid metabolism, and a decrease in vascular function in other tissues. Can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. Acts as a chemotactic factor for leukocyte populations expressing CMKLR1, particularly immature plasmacytoid dendritic cells, but also immature myeloid DCs, macrophages and natural killer cells. Exerts an anti-inflammatory role by preventing TNF/TNFA-induced VCAM1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-kappa-B and CRK/p38 through stimulation of AKT1/NOS3 signaling and nitric oxide production. Exhibits an antimicrobial function in the skin.
Alternative names
Retinoic acid receptor responder protein 2, Chemerin, Rarres2
What's included?
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Mouse Chemerin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Chemerin with a sensitivity of 1.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 14.06 - 900 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.75 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Mouse Serum | n 8 | mean - | SD - | C.V. 1.78 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Mouse Serum | n 3 | mean - | SD - | C.V. 2.49 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 86 - 101 % |
Sample type EDTA Plasma | Average % = 112 | Range 105 - 118 % |
Sample type Heparin Plasma | Average % = 102 | Range 100 - 106 % |
Sample type Citrate plasma | Average % = 92 | Range 92 - 93 % |
Sample type Cell culture media | Average % = 100 | Range 96 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Chemerin ELISA Kit (ab204520) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Chemerin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Chemerin with 1.75 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse Chemerin (also known as retinoic acid receptor responder protein 2, or Rarres2) is an adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). Chemerin positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Furthermore, Chemerin also acts as a proinflammatory adipokine, causing an increase in secretion of proinflammatory and prodiabetic adipokines.
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5 product images
Sandwich ELISA - Mouse Chemerin ELISA Kit (ab204520)
Mouse Chemerin standard curve comparison data.
Standard curve comparison between mouse Chemerin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Mouse Chemerin ELISA Kit (ab204520)
Example of the mouse Chemerin standard curve diluted in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Chemerin ELISA Kit (ab204520)
Linearity of dilution of mouse Chemerin in serum, stimulated L292 cultured media, and cell culture media.
Native mouse Chemerin was measured in 0.5% mouse serum and 0.66% PHA + PMA stimulated L929 cultured media that were diluted in a 2-fold dilution series in Sample Diluent NS. Recombinant mouse Chemerin was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Chemerin were measured in duplicate and interpolated from the mouse Chemerin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse Chemerin ELISA Kit (ab204520)
Linearity of dilution of mouse Chemerin in plasma samples.
Native mouse Chemerin was measured in 0.33% mouse plasma citrate, 0.5% mouse plasma EDTA and 0.66% mouse plasma heparin that were diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Chemerin were measured in duplicate and interpolated from the mouse Chemerin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse Chemerin ELISA Kit (ab204520)
Mouse Chemerin expression in L929 cells cultured in MEM with 10% horse serum and 100 µg/mL of Kanamycin.
During the exponential growth phase, L929 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10 ng/mL of PMA. Samples were diluted 1:300 in Sample Diluent NS. The concentrations of mouse Chemerin were interpolated from a standard curve diluted in Sample Diluent NS and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD). Note that media only gave an O.D. equivalent to background.
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