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AB246542

Mouse CHIT1 ELISA Kit

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Mouse CHIT1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CHIT1 with a sensitivity of 3.911 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Chitotriosidase-1, Chitinase-1, Chit1

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Sandwich ELISA - Mouse CHIT1 ELISA Kit (AB246542)
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Supplier Data

Sandwich ELISA - Mouse CHIT1 ELISA Kit (AB246542)

Interpolated concentrations of native CHIT1 in mouse tissue culture supernatant samples.

The concentrations of CHIT1 were measured in duplicate, interpolated from the CHIT1 standard curves and corrected for sample dilution. Undiluted spleen supernatant is 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHIT1 concentration was determined to be 422.68 pg/mL in spleen supernatant.

Sandwich ELISA - Mouse CHIT1 ELISA Kit (AB246542)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CHIT1 ELISA Kit (AB246542)

Interpolated concentrations of native CHIT1 in mouse serum and plasma.

The concentrations of CHIT1 were measured in duplicates, interpolated from the CHIT1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.5%, plasma (citrate) 0.5%, plasma (EDTA) 0.5%, and plasma (heparin) 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHIT1 concentration was determined to be 131.15 ng/mL in serum, 128.06 ng/mL in plasma (citrate), 105.87 ng/mL in plasma (EDTA), and 162.76 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse CHIT1 ELISA Kit (AB246542)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CHIT1 ELISA Kit (AB246542)

Example of mouse CHIT1 standard curve in Sample Diluent NS.

The CHIT1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, Tissue Culture Media, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 3.911 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CHIT1 ELISA Kit (ab246542) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CHIT1 protein in cit plasma, edta plasma, hep plasma, serum, and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CHIT1 with 3.911 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Serum", "range": "98 - 106 %", "average": "= 102" }, { "sample": "Tissue Culture Media", "range": "96 - 115 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "95 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "96 - 104 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "85 - 94 %", "average": "= 91" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHIT1 or chitotriosidase is a glycosyl hydrolase enzyme with significant roles in chitin breakdown. It has an approximate mass of 50 kDa and recognizes chitin-containing substances. CHIT1 predominantly expresses in macrophages and acts as an important enzyme in innate immunity by degrading pathologically accumulated chitin. Alternative names for CHIT1 include chitinase-1 and it is distinguishable by its active role in neutralizing fungal pathogens utilizing this chitinase mechanism.
Biological function summary

CHIT1 participates in inflammatory processes and helps in tissue remodeling which is important in immune responses. CHIT1 does not typically form part of a complex but operates independently to mediate macrophage response through its chitinase activity. It handles polysaccharide elements in cellular debris impacting macrophage activation states. Macrophages utilize CHIT1 to counteract foreign bodies engulfed during phagocytosis playing essential roles in managing fungal infections.

Pathways

CHIT1 influences both the immune response and metabolic pathways through its enzyme function. One key pathway involving CHIT1 is the mannose receptor-mediated clearance pathway which clears glycoproteins from systemic circulation. CHIT1 activity mirrors that of other chitinases like AMCase although they derive from separate genetic backgrounds. CHIT1 contribution enhances macrophage functionality within phagolysosomal pathways important for broad-spectrum pathogen eradication.

CHIT1 correlates significantly with Gaucher's disease and chronic obstructive pulmonary disease (COPD). In Gaucher's disease elevated CHIT1 levels serve as a biomarker indicating macrophage activation and infiltration. In the case of COPD CHIT1 modulates inflammatory responses that contribute to disease progression and respiratory complications. CHIT1 interacts with proteins associated with immune regulation such as TNF-alpha in these diseases impacting inflammatory pathways and disease severities.
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Product protocols

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Target data

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens (By similarity).
See full target information Chitotriosidase-1
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websiteProtocolBooklet
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