AB264631

Mouse Chromogranin A ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Mouse Chromogranin A ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Chromogranin A with a sensitivity of 93 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Chromogranin-A, CgA, Chga

2 Images

Sandwich ELISA - Mouse Chromogranin A ELISA Kit (AB264631)

sELISA

Supplier Data

Sandwich ELISA - Mouse Chromogranin A ELISA Kit (AB264631)

Interpolated concentrations of native Chromogranin A in mouse serum, plasma and cell culture supernatant samples.

The concentrations of Chromogranin A were measured in duplicates, interpolated from the Chromogranin A standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (citrate) 2%, plasma (EDTA) 1.5%, plasma (heparin) 2% and pancreas tissue culture supernatant 12%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Chromogranin A concentration was determined to be 367 ng/mL in neat serum, 256 ng/mL in neat plasma (citrate), 443 ng/mL in neat plasma (EDTA), 203 ng/mL in neat plasma (heparin) and 53 ng/mL in neat pancreas tissue culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Chromogranin A ELISA Kit (AB264631)

Example of mouse Chromogranin A standard curve in Sample Diluent NS.

The Chromogranin A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 93 pg/mL

Range

132.81 - 8500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse Chromogranin A ELISA Kit (ab264631) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Chromogranin A protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Chromogranin A with 93 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Chromogranin A (CHGA) a secreted acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. It is processed to number of non-overlapping peptides including Beta-Granin, Pancreastatin, Catestatin and various forms of Serpins. Chromogranin A is a useful serological and immunohistological marker for the presence of neuroendocrine tumors.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 113 %", "average": "= 103" }, { "sample": "Serum", "range": "93 - 107 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "94 - 111 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "74 - 100 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "110 - 123 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "96 - 109 %", "average": "= 101" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264631-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264631-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB264631-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264631-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Chromogranin A Capture Antibody", "number":"AB264631-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Chromogranin A Lyophilized Recombinant Protein", "number":"AB264631-CMP06", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264631-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264631-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264631-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Chromogranin A Detector Antibody", "number":"AB264631-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264631-CMP05", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.

Biological function summary

Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.

Pathways

Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.

Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.

style

left-column

Product protocols

style

left-column

Target data

Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas.. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Can induce mast cell migration, degranulation and production of cytokines and chemokines.. Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation (PubMed : 21436258). Pyroglutaminated (pGlu)-serpinin exerts an antiapoptotic effect on cells exposed to oxidative stress (PubMed : 21537909).

See full target information Chromogranin-A

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Chromogranin A ELISA Kit

Sandwich ELISA - Mouse Chromogranin A ELISA Kit (AB264631)

Sandwich ELISA - Mouse Chromogranin A ELISA Kit (AB264631)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise