Mouse Clusterin ELISA Kit (Apolipoprotein J) is a single-wash 90-min Simplestep used to quantify Mouse Clusterin (Apolipoprotein J) with a sensitivity of 280 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Publications
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- JCI insight 5:2020Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhanli Xie et. al.PubMed 32641582
- Alzheimer's research & therapy 11:422019Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSofía Fernández de Retana et. al.PubMed 31077261
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.78 - 50 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 280 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins (By similarity). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed:14741101). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity (By similarity). Following stress, promotes apoptosis (PubMed:12551933). Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. Following ER stress, suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. When secreted, does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (By similarity). When secreted, acts as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (PubMed:11865066).
Alternative names
Apoj, Msgp-2, Clu, Clusterin, Apolipoprotein J, Clustrin, Sulfated glycoprotein 2, Apo-J, SGP-2
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Mouse Clusterin ELISA Kit (Apolipoprotein J) is a single-wash 90-min Simplestep used to quantify Mouse Clusterin (Apolipoprotein J) with a sensitivity of 280 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.78 - 50 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 280 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 5 | C.V. 8.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 12.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 105 | Range 97 - 112 % |
Sample type Urine | Average % = 102 | Range 95 - 105 % |
Sample type Citrate plasma | Average % = 106 | Range 103 - 111 % |
Sample type Cell culture media | Average % = 99 | Range 95 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Clusterin ELISA Kit (Apolipoprotein J) (ab199079) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Clusterin (Apolipoprotein J) protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Clusterin (Apolipoprotein J) with 280 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Clusterin (also known as Apoliprotein J) is a multifunctional protein, named for its ability to induce cellular clustering. The secreted form is processed from a single chain into alpha and beta strands, that form a heterodimeric, 80 kDa protein. Clusterin is also present as an alternatively spliced, 50 kDa intracellular form isoform. As an extracellular chaperone, Clusterin prevents the aggregation of blood plasma proteins and inhibits amyloid fibrils. Clusterin also plays a role in both apoptosis and proliferation. Secreted Clusterin protects against apoptosis by the complement system, but can promote apoptosis when present in the nucleus. Intracellular Clusterin interacts with ubiquitin and the proteasomal machinery for target degradation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The biological target Apolipoprotein J also known as Clusterin or ApoJ is a glycoprotein with a mass of approximately 80 kDa. Clusterin is expressed in a wide range of tissues and bodily fluids including the brain liver and blood plasma. It acts as a chaperone helping to stabilize and transport proteins and lipids under physiological stress conditions. This protein also assists in inhibiting complement-mediated cell lysis thereby contributing to cell survival.
- Biological function summary
The multifunctional role of Clusterin involves cell aggregation apoptosis and tissue remodeling. It does not act alone; Clusterin can integrate into complex formation with other proteins influencing various cellular processes. It partners with lipoproteins in blood influencing lipid transport and metabolism. Its prominent presence in extracellular fluids suggests a significant role in modulating immune responses and reducing oxidative stress.
- Pathways
The involvement of Clusterin spans cholesterol metabolism and apoptotic pathways. It interacts importantly with key players like ApoE in cholesterol homeostasis impacting lipoprotein particle formation and hepatic lipid clearance. Additionally within apoptotic pathways Clusterin associates with Bcl-2 family proteins aiding in the regulation of cell death and survival signaling.
- Associated diseases and disorders
Apolipoprotein J correlates with Alzheimer's disease and certain cancers. In Alzheimer's Clusterin is upregulated associating with amyloid-beta plaques which implicates it in neurodegeneration processes. Additionally in cancer Clusterin connects with proteins like Bcl-xL influencing tumorigenesis and resistance to therapy. Its varied roles and interactions highlight its importance as a biomarker and potential therapeutic target.
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3 product images
Sandwich ELISA - Mouse Clusterin ELISA Kit (Apolipoprotein J) (ab199079)
Example of Clusterin standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Clusterin ELISA Kit (Apolipoprotein J) (ab199079)
Comparison of secreted Clusterin in unstimulated and PMA/PHA-stimulated L929 Cells.
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Clusterin was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the Clusterin Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 1.5 ng/mL unstimulated, 16.4 ng/mL stimulated. There was no detectable signal in media.
Sandwich ELISA - Mouse Clusterin ELISA Kit (Apolipoprotein J) (ab199079)
Linearity of dilution of the assay.
Interpolated concentrations of Clusterin in mouse serum and plasma (citrate). The concentrations of Clusterin were measured in duplicate and interpolated from the Clusterin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration was determined to be 59.1 μg/mL in mouse serum and 38.7 μg /mL in mouse plasma.
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