Mouse Clusterin ELISA Kit (Apolipoprotein J)

Product details

Mouse Clusterin ELISA Kit (Apolipoprotein J) (ab199079) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Clusterin (Apolipoprotein J) protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Clusterin (Apolipoprotein J) with 280 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Clusterin (also known as Apoliprotein J) is a multifunctional protein, named for its ability to induce cellular clustering. The secreted form is processed from a single chain into alpha and beta strands, that form a heterodimeric, 80 kDa protein. Clusterin is also present as an alternatively spliced, 50 kDa intracellular form isoform. As an extracellular chaperone, Clusterin prevents the aggregation of blood plasma proteins and inhibits amyloid fibrils. Clusterin also plays a role in both apoptosis and proliferation. Secreted Clusterin protects against apoptosis by the complement system, but can promote apoptosis when present in the nucleus. Intracellular Clusterin interacts with ubiquitin and the proteasomal machinery for target degradation.