Mouse Complement C3 ELISA Kit

Product details

Mouse Complement C3 ELISA Kit ab157711 is a sanwich ELISA to measure Mouse Complement factor 3 (C3) in Plasma and Serum with a sensitivity of 1.65 ng/mL.

How the assay works

In this assay the Complement C3 present in samples reacts with the anti- Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti- Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay specificity

Interferences:

These chemicals or biologicals will cause interferences in this assay causing compromised results or complete failure.

- Azide and thimerosal at concentrations higher than 0.1% inhibits the enzyme reaction.

Mouse Complement C3 ELISA Kit ab157711 protocol summary

1. Add standards or samples to the wells. Incubate for 20 min
2. Wash, then add 100µl of !x Enzyne-Antibody conjugate per well. Incubate for 20 min
3. Wash, then add 100 µl of TMB solution per well. Incubate 10 min in the dark
4. Add stop solution and read at 450 nm

How other researchers are using Mouse Complement C3 ELISA Kit ab157711

Mouse Complement C3 ELISA Kit ab157711 has been used in publications to evaluate C3 pathways as a therapeutic approach to membranous nephropathy ⁽¹⁾, to evaluate synthetic RNA viruses as a treatment for cancer ⁽²⁾, and to asses CR3 modulation of Asperigillus fumigatus ⁽³⁾.

References:

⁽¹⁾Nicola M Tomas et al. 2023 PMID: 36709213,
⁽²⁾Lorena Lerner et al. 2022 PMID: 36207308,
⁽³⁾Li Han et al. 2021 PMID: 34338598

Related and recommended products

Mouse Complement C3 ELISA Kit ab157711 is commonly used to study immune responses against infections as part of the complement system.

Other kits for measuring the complement system include:

- Mouse Complement C5a ELISA kit ab193718
- Mouse Complement C5 ELISA kit ab264609

These ELISA kits are also available for human:

- Human Complement C3 ELISA kit ab108823
- Human Complement C5a ELISA Kit ab193695
- Human Complement C5 ELISA Kit ab125963