AB193718

Mouse Complement C5a ELISA Kit

Name: Mouse Complement C5a ELISA Kit (ab193718) | Abcam
Brand: Abcam Limited
SKU: AB193718
Rating: 5 (1 reviews)

(1 Review)

(17 Publications)

Mouse Complement C5a ELISA Kit is a sandwich ELISA designed to quantify Mouse Complement C5a with a sensitivity of 1.5 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.5 - 400 pg/mL
- Cited in over 10 publications

View Alternative Names

Hc, C5, Complement C5, Hemolytic complement

2 Images

Sandwich ELISA - Mouse Complement C5a ELISA Kit (AB193718)

sELISA

Supplier Data

Sandwich ELISA - Mouse Complement C5a ELISA Kit (AB193718)

Typical Standard Curve in Assay Diluent A using ab193718 Complement C5a Mouse ELISA Kit.

sELISA

Supplier Data

Sandwich ELISA - Mouse Complement C5a ELISA Kit (AB193718)

Typical Standard Curve in Assay Diluent B using ab193718 Complement C5a Mouse ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.5 pg/mL

Range

1.5 - 400 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Complement C5a ELISA Kit ab193718 is a sandwich ELISA to measure Mouse Complement C5a in serum, plasma, cell culture supernatant with a sensitivity of 1.5 pg/ml.

How the assay works

This assay employs an antibody specific for mouse Complement C5a coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Complement C5a present in the samples. The wells are washed and biotinylated anti-mouse Complement C5a antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Complement C5a bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Complement C5a ELISA Kit ab193718 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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Recovery

[ { "sample": "Serum", "range": "127 - 140 %", "average": "= 133.4" }, { "sample": "Plasma", "range": "112 - 140 %", "average": "= 128.5" }, { "sample": "Cell culture media", "range": "121 - 147 %", "average": "= 132" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193718-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193718-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193718-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse C5a Microplate (12 strips x 8 wells)", "number":"AB193718-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193718-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse C5a detection antibody", "number":"AB193718-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse C5a Standard (Lyophilized)", "number":"AB193718-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193718-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193718-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB193718-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.

Biological function summary

The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.

Pathways

The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.

C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.

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Product protocols

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Target data

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

See full target information C5

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:104 PubMed40217267

2025

Moss-derived human complement factor H modulates retinal immune response and attenuates retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mandy Hector,Verena Behnke,Paulina Dabrowska-Schlepp,Andreas Busch,Andreas Schaaf,Thomas Langmann,Anne Wolf

Nature communications 15:10142 PubMed39587068

2024

Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa Titiz,Lorenzo Landini,Daniel Souza Monteiro de Araujo,Matilde Marini,Viola Seravalli,Martina Chieca,Pasquale Pensieri,Marco Montini,Gaetano De Siena,Benedetta Pasquini,Silvia Vannuccini,Luigi Francesco Iannone,Thiago M Cunha,Giulia Brancolini,Elisa Bellantoni,Irene Scuffi,Alessandra Mastricci,Martina Tesi,Mariarosaria Di Tommaso,Felice Petraglia,Pierangelo Geppetti,Romina Nassini,Francesco De Logu

Heliyon 10:e38139 PubMed39386804

2024

Deletion of Card9 eliminates the detrimental facets of mycobacterial adjuvants.

Applications

Unspecified application

Species

Unspecified reactive species

Hideo Mitsuyama,Ei'ichi Iizasa,Akiko Kukita,Shuji Toda,Hiroki Yoshida,Hiromasa Inoue,Hiromitsu Hara

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400819 PubMed38837628

2024

CD9 Counteracts Liver Steatosis and Mediates GCGR Agonist Hepatic Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zheng,Yuren Wang,Xin Xiong,Linlin Zhang,Jiaran Zhu,Bangliang Huang,Xiufei Liu,Jinbo Liu,Zhiming Zhu,Gangyi Yang,Hua Qu,Hongting Zheng

Biomaterials research 28:0017 PubMed38779139

2024

Development of Lipid Nanoparticle Formulation for the Repeated Administration of mRNA Therapeutics.

Applications

Unspecified application

Species

Unspecified reactive species

Yeji Lee,Michaela Jeong,Gyeongseok Lee,Jeongeun Park,Hyein Jung,Seongeun Im,Hyukjin Lee

Journal of virology 97:e0055623 PubMed37191498

2023

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Gi Uk Jeong,Sumin Lee,Do Yeon Kim,Jaemyun Lyu,Gun Young Yoon,Kyun-Do Kim,Keun Bon Ku,Junsu Ko,Young-Chan Kwon

Advanced therapeutics 4: PubMed34095458

2021

Synthetic platelet microgels containing fibrin knob B mimetic motifs enhance clotting responses.

Applications

Unspecified application

Species

Unspecified reactive species

Seema Nandi,Emily Mihalko,Kimberly Nellenbach,Mario Castaneda,John Schneible,Mary Harp,Halston Deal,Michael Daniele,Stefano Menegatti,Thomas H Barker,Ashley C Brown

Frontiers in immunology 11:603924 PubMed33613523

2021

Infection Induces Sustained Up-Regulation of Complement Factor B and C5a Receptor in the Mouse Brain Microglial Activation: Implication for the Alternative Complement Pathway Activation and Anaphylatoxin Signaling in Cerebral Toxoplasmosis.

Applications

Unspecified application

Species

Unspecified reactive species

Noriko Shinjyo,Kenji Hikosaka,Yasutoshi Kido,Hiroki Yoshida,Kazumi Norose

Journal of cellular and molecular medicine 25:960-974 PubMed33280239

2020

Complement C5 activation promotes type 2 diabetic kidney disease via activating STAT3 pathway and disrupting the gut-kidney axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Li,Tiantian Wei,Shuyun Liu,Chengshi Wang,Meng Zhao,Yanhuan Feng,Liang Ma,Yanrong Lu,Ping Fu,Jingping Liu

Breast cancer research : BCR 22:103 PubMed32993785

2020

Sunitinib facilitates metastatic breast cancer spreading by inducing endothelial cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Denian Wang,Fei Xiao,Zhongxue Feng,Min Li,Lingmiao Kong,Luping Huang,Yong'gang Wei,Hongyu Li,Fei Liu,Haili Zhang,Wei Zhang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Complement C5a ELISA Kit

Sandwich ELISA - Mouse Complement C5a ELISA Kit (AB193718)

Sandwich ELISA - Mouse Complement C5a ELISA Kit (AB193718)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise