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AB285231

Mouse Creatine Kinase MB ELISA Kit

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(7 Publications)

Mouse Creatine Kinase MB ELISA Kit is a sandwich ELISA designed to quantify Mouse Creatine Kinase MB with a sensitivity of 46.825 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 78.125 - 5000 pg/mL

View Alternative Names

Ckmm, Ckm, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

1 Images
Sandwich ELISA - Mouse Creatine Kinase MB ELISA Kit (AB285231)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Creatine Kinase MB ELISA Kit (AB285231)

Standard CK-MB Curve.

Typical Standard Curve : This standard curve is for demonstration only. A standard curve must be run with each assay

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 46.825 pg/mL

Range

78.125 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Creatine Kinase MB ELISA Kit ab285231 is a sandwich ELISA to measure Mouse Creatine Kinase MB in serum, plasma, tissue homogenate, other biological fluids with a sensitivity of 46.825 pg/ml.

How the assay works

The density of color is proportional to the amount of CK-MB captured from the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Creatine Kinase MB ELISA Kit ab285231 protocol summary

1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285231-96Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285231-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285231-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample / Standard Dilution Buffer", "number":"AB285231-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285231-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285231-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285231-CMP02", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285231-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285231-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285231-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285231-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285231-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
See full target information Ckm
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 105:e70106 PubMed40556480

2025

Artesunate Inhibits Myocardial Ischemia Reperfusion Injury via Downregulation of Lysine Specific Demethylase 5A.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Liu,Pengtao Zou,Yanmei Chen,Juanying Li,Qiang Liu,Qing Shangguan

Journal of translational medicine 22:983 PubMed39482719

2024

Hyperuricemia suppresses lumican, exacerbating adverse remodeling after myocardial infarction by promoting fibroblast phenotype transition.

Applications

Unspecified application

Species

Unspecified reactive species

Zehao Zhuang,Ao Liu,Jinghong Zhang,Shuangjian Han,Lu Tang,Tingting Yu,Yiping Shi,Hui Li,Heng Yang,Peiyuan Bai,Yanhua Tang

Allergologia et immunopathologia 52:21-28 PubMed39278847

2024

extract relieved sepsis-induced myocardial ferroptosis and inflammation by inhibiting lncRNA MIAT/TRAF6/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Shi,Xiaobo Yang,Hong Jiang,Shanxia Wu,Yan Hong,Wei Su,Xuan Wang

Environmental toxicology 39:1682-1699 PubMed38041472

2023

Phenylacetyl glutamine (PAGln) enhances cardiomyocyte death after myocardial infarction through β1 adrenergic receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Xu,Na Yang,Bao-Yan Wang,Lin Zhou,Li-Li Xu,Yan Chen,Dong-Jin Wang,Wei-Hong Ge

International journal of biological sciences 19:3428-3440 PubMed37497006

2023

Lats2 deficiency protects the heart against myocardial infarction by reducing inflammation and inhibiting mitochondrial fission and STING/p65 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Libao Liu,Shuai Huang,Yingzhen Du,Hao Zhou,Kai Zhang,Jinyuan He

American journal of translational research 15:3928-3941 PubMed37434842

2023

MALAT1 knockdown alleviates myocardial injury in mice with severe acute pancreatitis via the miR-374a/Sp1/Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Renlian Gu,Zhong Shen,Yanshuo Ye

Frontiers in molecular biosciences 9:1008802 PubMed36304927

2022

Exosomes derived from M2 macrophages induce angiogenesis to promote wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Leifeng Lyu,Yuanqing Cai,Guangyang Zhang,Zhaopu Jing,Jialin Liang,Rupeng Zhang,Xiaoqian Dang,Chen Zhang
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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