Mouse CSF-1-R ELISA Kit, colorimetric, 90-min ELISA (ab240681)

Mouse CSF-1-R ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CSF-1-R with a sensitivity of 0.04 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Tissue, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Mouse
Range: 0.16 - 10 ng/mL
Assay time: 1h 30m
Sensitivity: = 0.04 ng/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

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Target data

See full target information Csf1r

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity).

Alternative names

CD115, Csfmr, Fms, Csf1r, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R

What's included?

1 x 384 Tests

Components

10X Mouse CSF-1-R Capture Antibody

1 x 600 µL

10X Mouse CSF-1-R Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

Antibody Diluent CPR2

1 x 6 mL

Mouse CSF-1-R Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 250 mL

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Tissue, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Mouse
Range: 0.16 - 10 ng/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 0.04 ng/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Serum	n 8	mean -	SD -	C.V. 4.8

Inter assay

Sample	n	mean	SD	C.V.
Sample Serum	n 3	mean -	SD -	C.V. 4

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 92	Range 85 - 97 %
Sample type Serum	Average % = 108	Range 102 - 111 %
Sample type EDTA Plasma	Average % = 102	Range 95 - 109 %
Sample type Cell culture extracts	Average % = 118	Range 116 - 119 %
Sample type Heparin Plasma	Average % = 105	Range 101 - 108 %
Sample type Citrate plasma	Average % = 108	Range 106 - 111 %
Sample type Tissue Extracts	Average % = 118	Range 111 - 132 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Mouse CSF-1-R ELISA Kit (ab240681) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSF-1-R protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CSF-1-R with 0.04 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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9 product images

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Example of mouse CSF-1-R standard curve in Sample Diluent NS.
The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Example of mouse CSF-1-R standard curve in 1X Cell Extraction Buffer PTR.
The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Interpolated concentrations of native CSF-1-R in mouse serum, plasma (EDTA), plasma (heparin), and plasma (citrate) samples.
The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:3,200, plasma (EDTA) 1:3,200, plasma (heparin) 1:3,200, and plasma (citrate) 1:1,600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 6.8 μg/mL in serum, 6.9 μg/mL in plasma (EDTA), 6.8 μg/mL in plasma (heparin), and 3.9 μg/mL in plasma (citrate).
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Interpolated concentrations of native CSF-1-R in mouse spleen cell supernatant, liver cell supernatant, and RAW cell supernatant samples.
The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen cell supernatant 2.5%, liver cell supernatant 25%, and RAW cell supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 78.9 ng/mL in neat spleen cell supernatant, 13 ng/mL in neat liver cell supernatant, and 1,024 ng/mL in neat RAW cell supernatant.
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Interpolated concentrations of native CSF-1-R in mouse spleen cell extract and RAW 264.7 cell extract samples.
The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen cell extract 75 μg/mL and RAW264.7 cell extract 37.5 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Interpolated concentrations of mouse CSF-1-R in serum in 96-well vs. 384-well plates.
Interpolated concentration of native CSF-1-R was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:3,200 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Example of mouse CSF-1-R standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.
Example of mouse CSF-1-R standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Example of mouse CSF-1-R standard curve in Sample Diluent NS in 96-well vs. 384-well plate.
Example of mouse CSF-1-R standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)
Interpolated concentrations of mouse CSF-1-R in RAW264.7 cell extract in 96-well vs. 384-well plates.
Interpolated concentration of native CSF-1-R was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50 µg/mL RAW264.7 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.