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AB240681

Mouse CSF-1-R ELISA Kit

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(1 Publication)

Mouse CSF-1-R ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CSF-1-R with a sensitivity of 0.04 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD115, Csfmr, Fms, Csf1r, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R

10 Images
ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • ELISA

Supplier Data

ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Unknown

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Interpolated concentrations of native CSF-1-R in mouse spleen cell extract and RAW 264.7 cell extract samples.

The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows : spleen cell extract 75 μg/mL and RAW264.7 cell extract 37.5 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Unknown

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Interpolated concentrations of native CSF-1-R in mouse serum, plasma (EDTA), plasma (heparin), and plasma (citrate) samples.

The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 3,200, plasma (EDTA) 1 : 3,200, plasma (heparin) 1 : 3,200, and plasma (citrate) 1 : 1,600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 6.8 μg/mL in serum, 6.9 μg/mL in plasma (EDTA), 6.8 μg/mL in plasma (heparin), and 3.9 μg/mL in plasma (citrate).

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Unknown

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Example of mouse CSF-1-R standard curve in 1X Cell Extraction Buffer PTR.

The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Unknown

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Interpolated concentrations of native CSF-1-R in mouse spleen cell supernatant, liver cell supernatant, and RAW cell supernatant samples.

The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows : spleen cell supernatant 2.5%, liver cell supernatant 25%, and RAW cell supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 78.9 ng/mL in neat spleen cell supernatant, 13 ng/mL in neat liver cell supernatant, and 1,024 ng/mL in neat RAW cell supernatant.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Unknown

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Example of mouse CSF-1-R standard curve in Sample Diluent NS.

The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Lab

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Example of mouse CSF-1-R standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

Example of mouse CSF-1-R standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Lab

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Interpolated concentrations of mouse CSF-1-R in serum in 96-well vs. 384-well plates.

Interpolated concentration of native CSF-1-R was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 3,200 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Lab

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Interpolated concentrations of mouse CSF-1-R in RAW264.7 cell extract in 96-well vs. 384-well plates.

Interpolated concentration of native CSF-1-R was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50 µg/mL RAW264.7 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)
  • sELISA

Lab

Sandwich ELISA - Mouse CSF-1-R ELISA Kit (AB240681)

Example of mouse CSF-1-R standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.

Example of mouse CSF-1-R standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.04 ng/mL

Range

0.16 - 10 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Secondary Antibodies

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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  • 5
  • 67
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CSF-1-R ELISA Kit ab240681 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse CSF-1-R in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue. This SimpleStep sensitivity is 0.04 ng/mL.

How the assay works

Mouse CSF-1-R SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse CSF-1-R ELISA Kit ab240681 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse CSF-1-R ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CSF-1-R antibody [EPR21885-208] - BSA and Azide free (Capture) ab245093
- Anti-CSF-1-R antibody [EPR21885-148] - BSA and Azide free (Detector) ab245093

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 97 %", "average": "= 92" }, { "sample": "Serum", "range": "102 - 111 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "95 - 109 %", "average": "= 102" }, { "sample": "Cell culture extracts", "range": "116 - 119 %", "average": "= 118" }, { "sample": "Heparin Plasma", "range": "101 - 108 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "106 - 111 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "111 - 132 %", "average": "= 118" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity).
See full target information Csf1r
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 26:416-429 PubMed36635496

2023

A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Bettina van Lengerich,Lihong Zhan,Dan Xia,Darren Chan,David Joy,Joshua I Park,David Tatarakis,Meredith Calvert,Selina Hummel,Steve Lianoglou,Michelle E Pizzo,Rachel Prorok,Elliot Thomsen,Laura M Bartos,Philipp Beumers,Anja Capell,Sonnet S Davis,Lis de Weerd,Jason C Dugas,Joseph Duque,Timothy Earr,Kapil Gadkar,Tina Giese,Audrey Gill,Johannes Gnörich,Connie Ha,Malavika Kannuswamy,Do Jin Kim,Sebastian T Kunte,Lea H Kunze,Diana Lac,Kendra Lechtenberg,Amy Wing-Sze Leung,Chun-Chi Liang,Isabel Lopez,Paul McQuade,Anuja Modi,Vanessa O Torres,Hoang N Nguyen,Ida Pesämaa,Nicholas Propson,Marvin Reich,Yaneth Robles-Colmenares,Kai Schlepckow,Luna Slemann,Hilda Solanoy,Jung H Suh,Robert G Thorne,Chandler Vieira,Karin Wind-Mark,Ken Xiong,Y Joy Yu Zuchero,Dolo Diaz,Mark S Dennis,Fen Huang,Kimberly Scearce-Levie,Ryan J Watts,Christian Haass,Joseph W Lewcock,Gilbert Di Paolo,Matthias Brendel,Pascal E Sanchez,Kathryn M Monroe
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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