JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326434

Mouse CSF-1-R ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse CSF-1-R ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse CSF-1-R with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 41.15 - 90000 pg/ml with a sensitivity of 12.51pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

CD115, Csfmr, Fms, Csf1r, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R

6 Images
Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)
  • sELISA

Lab

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)
  • sELISA

Lab

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)

Standard curve comparison between the Chemiluminescent Mouse CSF-1-R SimpleStep ELISA and colorimetric Mouse CSF-1-R SimpleStep ELISA (ab240681) in Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)
  • sELISA

Lab

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)

Dynamic range comparison between the Chemiluminescent Mouse CSF-1-R SimpleStep ELISA and colorimetric Mouse CSF-1-R SimpleStep ELISA (ab240681). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)
  • sELISA

Unknown

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)

Interpolated concentrations of native CSF-1-R in mouse serum plasma (EDTA) plasma (heparin) and plasma (citrate) samples.

The concentrations of CSF-1-R were measured in duplicates interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 3200 plasma (EDTA) 1 : 3200 plasma (heparin) 1 : 3200 and plasma (citrate) 1 : 1600. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean CSF-1-R concentration was determined to be 6.8 µg/mL in serum 6.9 µg/mL in plasma (EDTA) 6.8 µg/mL in plasma (heparin) and 3.9 µg/mL in plasma (citrate).

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)
  • sELISA

Unknown

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)

Interpolated concentrations of native CSF-1-R in mouse spleen cell extract and RAW 264.7 cell extract samples.

The concentrations of CSF-1-R were measured in duplicates interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows : spleen cell extract 75 µg/mL and RAW264.7 cell extract 37.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2).

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)
  • sELISA

Unknown

Sandwich ELISA - Mouse CSF-1-R ELISA Kit, Chemiluminescent (AB326434)

Interpolated concentrations of native CSF-1-R in mouse spleen cell supernatant liver cell supernatant and RAW cell supernatant samples.

The concentrations of CSF-1-R were measured in duplicates interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows : spleen cell supernatant 2.5% liver cell supernatant 25% and RAW cell supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean CSF-1-R concentration was determined to be 78.9 ng/mL in neat spleen cell supernatant 13 ng/mL in neat liver cell supernatant and 1024 ng/mL in neat RAW cell supernatant.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Cell culture extracts, Heparin Plasma, Citrate plasma, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 12.51 pg/mL

Range

41.15 - 90000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse CSF-1-R ELISA Kit, Chemiluminescent (ab326434) is a rapid single-wash sandwich 55-min ELISA to measure Mouse CSF-1-R in Cell culture supernatant, Serum, EDTA Plasma, Cell culture extracts, Heparin Plasma, Citrate plasma, Tissue Extracts with a sensitivity of 12.51 pg/mL.

How the assay works

Mouse CSF-1-R ELISA Kit, Chemiluminescent (ab326434) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse CSF-1-R (ab326434) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Mouse CSF1R/ MCSFR1 antibody [EPR21885-208] - BSA and Aside free (Capture) ab244820
- Anti-Mouse CSF1R/ MCSFR1 antibody [EPR21885-148] - BSA and Aside free (Detector) ab245093
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 97 %", "average": "= 92" }, { "sample": "Serum", "range": "102 - 111 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "95 - 109 %", "average": "= 102" }, { "sample": "Cell culture extracts", "range": "116 - 119 %", "average": "= 118" }, { "sample": "Heparin Plasma", "range": "101 - 108 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "106 - 111 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "111 - 132 %", "average": "= 118" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326434-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326434-CMP11", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326434-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326434-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB326434-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326434-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CSF-1-R Lyophilized Recombinant Protein", "number":"AB326434-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326434-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326434-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CSF-1-R Detector Antibody", "number":"AB326434-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326434-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CSF-1-R Capture Antibody", "number":"AB326434-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity).
See full target information Csf1r
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com