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AB155453

Mouse CT-1 ELISA Kit

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Mouse CT-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse CT-1 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Cardiotrophin-1, CT-1, Ctf1

1 Images
Sandwich ELISA - Mouse CT-1 ELISA Kit (AB155453)
  • sELISA

Unknown

Sandwich ELISA - Mouse CT-1 ELISA Kit (AB155453)

CT-1 Mouse ELISA Kit.

Representative standard curve using ab155453

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 0.2 ng/mL

Range

0.25 - 60 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CT-1 Mouse ELISA Kit (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Mouse CT-1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for CT-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CT-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CT-1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CT-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "70 - 88 %", "average": "= 78.13" }, { "sample": "Plasma", "range": "73 - 96 %", "average": "= 83.28" }, { "sample": "Cell culture media", "range": "84 - 100 %", "average": "= 92.96" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155453-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155453-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155453-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CT-1 Microplate (12 x 8 wells)", "number":"AB155453-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB155453-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155453-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse CT-1", "number":"AB155453-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse CT-1 Standard (lyophilized)", "number":"AB155453-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155453-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155453-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
See full target information Ctf1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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