JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB289838

Mouse CTGF ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse CTGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse CTGF in Mouse in Biofluids samples.

View Alternative Names

betaIG-M2, Ctgf, Fisp-12, Fisp12, Hcs24, Ccn2, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Protein FISP-12

1 Images
Functional Studies - Mouse CTGF ELISA Kit (AB289838)
  • FuncS

Supplier Data

Functional Studies - Mouse CTGF ELISA Kit (AB289838)

Mouse CTGF ELISA Kit.

Typical standard curve for reference purposes only.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.1 ng/mL

Range

0.16 - 10 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse CTGF ELISA Kit (ab289838, E4838) is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of CTGF bound.
style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.21" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab289838-96Test", "assetComponentDetails": [ { "size":"1 x 14 mL", "name":"HRP Conjugate Diluent", "number":"AB289838-CMP09" }, { "size":"1 x 20 mL", "name":"Reference Standard & Sample Diluent", "number":"AB289838-CMP05" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB289838-CMP02" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB289838-CMP07" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB289838-CMP11" }, { "size":"1 x 10 mL", "name":"Substrate Reagent", "number":"AB289838-CMP10" }, { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB289838-CMP06" }, { "size":"2 x 1 Vial", "name":"Reference Standard", "number":"AB289838-CMP04" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB289838-CMP03" }, { "size":"1 x 120 µL", "name":"HRP Conjugate (100x)", "number":"AB289838-CMP08" }, { "size":"1 x 120 µL", "name":"Biotinylated Detection Ab (100x)", "number":"AB289838-CMP01" } ] }, "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab289838-96Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB289838-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB289838-CMP07", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB289838-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB289838-CMP03", "productcode":"" }, { "size":"1 x 14 mL", "name":"HRP Conjugate Diluent", "number":"AB289838-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP Conjugate (100x)", "number":"AB289838-CMP08", "productcode":"" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB289838-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Reference Standard", "number":"AB289838-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotinylated Detection Ab (100x)", "number":"AB289838-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Reference Standard & Sample Diluent", "number":"AB289838-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"Substrate Reagent", "number":"AB289838-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTGF also known as Connective Tissue Growth Factor is a significant protein in the CCN family involved in regulating various cellular functions. CTGF has a molecular weight of approximately 36-38 kDa. It is highly expressed in tissues such as the skin kidney and vascular tissues. This protein is secreted and can be found in the extracellular matrix where it interacts with other cellular components to mediate its effects.
Biological function summary

Connective Tissue Growth Factor plays an important role in cell adhesion proliferation and differentiation. It operates as an independent protein but can also be part of the extracellular matrix structure. CTGF functions as a mediator in the interaction between cells and their surrounding environment influencing processes such as angiogenesis and wound healing through these interactions.

Pathways

CTGF fits into the TGF-beta and Wnt signaling pathways where it helps modulate cellular responses to external stimuli. It interacts with proteins like fibronectin and integrins within these pathways enhancing cellular communication and response. These pathways are fundamental for both development and pathological conditions showing CTGF's influence on various biological processes.

CTGF shows a strong connection with fibrosis and cancer. In fibrotic conditions the CTGF protein interacts with collagen and fibronectin leading to excessive deposition in tissues. In cancers CTGF may promote tumor progression by facilitating angiogenesis and promoting invasive behaviors in tumors. These interactions highlight CTGF's role as a target for therapeutic strategies aimed at treating these diseases.
style
left-column

Product protocols

style
left-column

Target data

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).
See full target information Ccn2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684207

2024

Endothelial Dysfunction and Impaired Wound Healing Following Radiation Combined Skin Wound Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Bin Lin,Min Zhai,Lisa Hull,Wanchang Cui,Mang Xiao
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB210579

Mouse Pro-Collagen I alpha 1 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

  • 5
  • 1
Assay Kits

AB253223

Mouse CYR61 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse CYR61 ELISA Kit (AB253223)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB210967

Mouse Fibronectin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Fibronectin ELISA Kit (AB210967)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com