Mouse CTGF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse CTGF in Mouse in Plasma, Serum, Other biological fluids samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.16 - 10 ng/mL
- Sensitivity
- = 0.1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).
Alternative names
betaIG-M2, Ctgf, Fisp-12, Fisp12, Hcs24, Ccn2, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Protein FISP-12
What's included?
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Mouse CTGF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse CTGF in Mouse in Plasma, Serum, Other biological fluids samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.16 - 10 ng/mL
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 0.1 ng/mL
- General recovery
- 93.6%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 5.21 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Mouse CTGF ELISA Kit (ab289838, E4838) is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of CTGF bound.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CTGF also known as Connective Tissue Growth Factor is a significant protein in the CCN family involved in regulating various cellular functions. CTGF has a molecular weight of approximately 36-38 kDa. It is highly expressed in tissues such as the skin kidney and vascular tissues. This protein is secreted and can be found in the extracellular matrix where it interacts with other cellular components to mediate its effects.
- Biological function summary
Connective Tissue Growth Factor plays an important role in cell adhesion proliferation and differentiation. It operates as an independent protein but can also be part of the extracellular matrix structure. CTGF functions as a mediator in the interaction between cells and their surrounding environment influencing processes such as angiogenesis and wound healing through these interactions.
- Pathways
CTGF fits into the TGF-beta and Wnt signaling pathways where it helps modulate cellular responses to external stimuli. It interacts with proteins like fibronectin and integrins within these pathways enhancing cellular communication and response. These pathways are fundamental for both development and pathological conditions showing CTGF's influence on various biological processes.
- Associated diseases and disorders
CTGF shows a strong connection with fibrosis and cancer. In fibrotic conditions the CTGF protein interacts with collagen and fibronectin leading to excessive deposition in tissues. In cancers CTGF may promote tumor progression by facilitating angiogenesis and promoting invasive behaviors in tumors. These interactions highlight CTGF's role as a target for therapeutic strategies aimed at treating these diseases.
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1 product image
Functional Studies - Mouse CTGF ELISA Kit (ab289838)
Mouse CTGF ELISA Kit.
Typical standard curve for reference purposes only.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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