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AB113346

Mouse CTLA 4 ELISA Kit

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Mouse CTLA 4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse CTLA 4 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD152, Cd152, Ctla4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4

2 Images
Sandwich ELISA - Mouse CTLA 4 ELISA Kit (AB113346)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CTLA 4 ELISA Kit (AB113346)

Representative Standard Curve using ab113346

Sandwich ELISA - Mouse CTLA 4 ELISA Kit (AB113346)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CTLA 4 ELISA Kit (AB113346)

Representative Standard Curve using ab113346

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

4.92 - 1200 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CTLA4 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CTLA4 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse CTLA4 coated on a 96- well plate. Standards and samples are pipetted into the wells and CTLA4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CTLA4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CTLA4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "67 - 89 %", "average": "= 76.34" }, { "sample": "Serum", "range": "101 - 114 %", "average": "= 107.3" }, { "sample": "Plasma", "range": "100 - 110 %", "average": "= 104.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113346-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113346-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113346-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB113346-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113346-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB113346-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CTLA4 Microplate (12 strips x 8 wells)", "number":"AB113346-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse CTLA4", "number":"AB113346-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113346-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse CTLA4 Standard (lyophilized)", "number":"AB113346-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTLA-4 also known as Cytotoxic T-Lymphocyte Antigen 4 or CD152 is a protein receptor with an approximate molecular weight of 25–37 kDa. Expressed primarily on the surface of T cells CTLA-4 acts as an immune checkpoint molecule. It competes with the costimulatory receptor CD28 for binding to CD80/CD86 ligands on antigen-presenting cells. This competitive binding results in an inhibitory signal that reduces T cell activation and proliferation.
Biological function summary

CTLA-4 modulates immune responses by serving as a critical negative regulator in T cell activation. Unlike CD28 which promotes T cell responses CTLA-4 inhibits these responses helping to maintain immune homeostasis. CTLA-4 exists in a monomeric form on the cell surface but can form a dimeric complex when interacting with ligands. By dampening the immune response CTLA-4 prevents excessive tissue damage during immune reactions.

Pathways

CTLA-4 is integral in immune signaling pathways specifically the pathway that involves T cell regulation. It functions within the context of the immune synapse influencing the balance between activation and inhibition of T cells. CTLA-4 engages with proteins like CD28 and PD-1 in these pathways each playing distinct roles in modulating immune responses.

CTLA-4 has critical connections to autoimmune diseases and cancer. In autoimmune diseases CTLA-4 dysfunction may lead to improper immune regulation potentially resulting in conditions such as rheumatoid arthritis. In cancer CTLA-4-related proteins like PD-1 can be manipulated by tumors to escape immune detection making CTLA-4 a target for immunotherapy interventions. Understanding CTLA-4 interactions with CD28 and PD-1 has led to the development of therapies aiming to modulate immune responses in these diseases.
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Product protocols

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Target data

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
See full target information Ctla4
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websiteProtocolBooklet
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