Mouse CTLA-4 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse CTLA-4 in Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
31.25 - 2000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 14.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Alternative names
Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, Cd152, Ctla4
What's included?
Recommended products
Mouse CTLA-4 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse CTLA-4 in Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
31.25 - 2000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 14.5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 1.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 105 | Range 100 - 108 % |
Sample type EDTA Plasma | Average % = 104 | Range 100 - 108 % |
Sample type Tissue Extracts | Average % = 91 | Range 89 - 93 % |
Sample type Cell culture media | Average % = 98 | Range 97 - 100 % |
Sample type Heparin Plasma | Average % = 105 | Range 100 - 110 % |
Sample type Citrate plasma | Average % = 95 | Range 93 - 97 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse CTLA-4 ELISA Kit (ab255720) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CTLA-4 protein in cell culture media, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CTLA-4 with 14.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cytotoxic T lymphocyte antigen-4 (CTLA-4) is a transmembrane protein expressed on T regulator cells. CTLA-4 is a negative regulator of T cell activation. CTLA-4 binds markers on antigen presenting cells (APC) including B7, which functions as an immune check point to down regulate the immune response. Blocking CTLA-4 allows for activation and proliferation of more T cells which reduces the mediated immunosuppression. CTLA-4 soluble extracellular domain in serum and plasma is an indication of autoimmune diseases. ab255720 is recognizes the extracellular domain of this transmembrane protein.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CTLA-4 also known as CD152 is an immune checkpoint receptor with a molecular weight of approximately 34 kDa. This protein is expressed on the surface of T-cells especially after activation and sometimes on regulatory T-cells (Tregs). CTLA-4 competes with CD28 for binding to the same ligands CD80 and CD86 on antigen-presenting cells. Unlike CD28 which stimulates T-cell activation CTLA-4 sends inhibitory signals to downregulate immune responses. By doing this CTLA-4 helps maintain immune system homeostasis and prevents autoimmune reactions.
- Biological function summary
The CTLA-4 protein functions as a critical regulator of the immune system. It interacts with CD80/CD86 in a complex that effectively transmits inhibitory signals to T-cells. CTLA-4 controls the amplitude of the initial activation of T-cells ensuring that the body's immune responses are kept under control. In regulatory T-cells CTLA-4 engagement contributes to their immunosuppressive functions through which they maintain tolerance to self-antigens and prevent overactive immune reactions.
- Pathways
CTLA-4 involves itself in the adaptive immune response pathways specifically the regulation of T-cell activity. CTLA-4's interaction with CD80/CD86 modulates pathways associated with TCR (T-cell receptor) signaling. When CTLA-4 binds its ligands it recruits phosphatases such as SHP-2 that dephosphorylate signaling proteins leading to the downregulation of T-cell interaction. This pathway interaction exemplifies how CTLA-4 acts in concert with proteins like CD28 to finely tune immune responses.
- Associated diseases and disorders
CTLA-4 plays a role in autoimmune diseases and cancer. The dysregulation of CTLA-4 function can lead to autoimmune disorders where the immune system attacks the body's own tissue. In cancer tumor cells may manipulate CTLA-4 pathways to evade immune surveillance. Targeted therapies like anti-CTLA-4 antibodies such as ipilimumab have been developed to block CTLA-4 aiming to enhance the immune system’s ability to attack cancer cells. These treatments highlight the role of CTLA-4 in balancing immune activity and the potential to alter this balance for therapeutic benefit.
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4 product images
Sandwich ELISA - Mouse CTLA-4 ELISA Kit (ab255720)
Example of mouse CTLA-4 standard curve in Sample Diluent NS.
The CTLA-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CTLA-4 ELISA Kit (ab255720)
Interpolated concentrations of native CTLA-4 in mouse thymus extract sample based on a 1,000 μg/mL extract load.
The concentrations of CTLA-4 were measured in duplicate and interpolated from the CTLA-4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTLA-4 concentration was determined to be 193 pg/mL in thymus extract.
Sandwich ELISA - Mouse CTLA-4 ELISA Kit (ab255720)
Interpolated concentrations of spike CTLA-4 in mouse serum and plasma samples.
The concentrations of CTLA-4 were measured in duplicates, interpolated from the CTLA-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse CTLA-4 ELISA Kit (ab255720)
Interpolated concentrations of spike CTLA-4 in mouse cell culture media samples.
The concentrations of CTLA-4 were measured in duplicates, interpolated from the CTLA-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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