Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) is a single-wash 90-min Simplestep used to quantify Mouse CXCL1 (GRO alpha) with a sensitivity of 0.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
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Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.7 - 2800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation (By similarity). Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity.
Alternative names
Gro, Gro1, Mgsa, Scyb1, Cxcl1, Growth-regulated alpha protein, C-X-C motif chemokine 1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51
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Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) is a single-wash 90-min Simplestep used to quantify Mouse CXCL1 (GRO alpha) with a sensitivity of 0.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.7 - 2800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Mouse Serum | n 8 | mean - | SD - | C.V. 6.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Mouse Serum | n 3 | mean - | SD - | C.V. 10.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 110 | Range 109 - 110 % |
Sample type Serum | Average % = 91 | Range 88 - 94 % |
Sample type EDTA Plasma | Average % = 94 | Range 89 - 98 % |
Sample type Cell culture extracts | Average % = 95 | Range 95 - 96 % |
Sample type Heparin Plasma | Average % = 79 | Range 72 - 90 % |
Sample type Citrate plasma | Average % = 94 | Range 92 - 97 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
CXCL1 in vitro CatchPoint® ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is designed for the quantitative measurement of CXCL1 (GRO alpha) protein in mouse serum, plasma, cell culture supernatants, and cell extract samples.
This CatchPoint ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint® ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint® HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
CXCL1 is a member of the CXC family of chemokines. Chemokines play roles in normal and pathological processes including allergic responses, angiogenesis, inflammation, tumor growth and metastasis. Mouse CXCL2 and CXCL3 share 67% and 60% sequence homology with mouse CXCL1, respectively. Additionally, Rat CXCL1 is 89% homologous with mouse CXCL1.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.
- Biological function summary
CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.
- Pathways
CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.
- Associated diseases and disorders
CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.
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5 product images
Sandwich ELISA - Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) (ab229426)
CXCL1 (GRO alpha) standard curve in Sample Diluent NS.
The CXCL1 (GRO alpha) standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) (ab229426)
Interpolated concentrations of spiked CXCL1 (GRO alpha) in mouse serum and plasma samples.
The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 386.84 pg/mL in neat serum, 399.45 pg/mL in neat plasma (citrate), 364.34 pg/mL in neat plasma (EDTA), and 505.80 pg/mL in neat plasma (heparin).
Sandwich ELISA - Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) (ab229426)
Interpolated concentrations of native CXCL1 (GRO alpha) in mouse serum and plasma samples.
The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 48.56 pg/mL in neat serum, 24.34 pg/mL in neat plasma (citrate), 23.67 pg/mL in neat plasma (EDTA), and 176.56 pg/mL in neat plasma (heparin).
Sandwich ELISA - Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) (ab229426)
Interpolated concentrations of native CXCL1 (GRO alpha) in mouse NIH 3T3 cell culture supernatant sample.
The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: NIH 3T3 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 472.88 pg/mL in neat mouse NIH 3T3 cell culture supernatant.
Sandwich ELISA - Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) (ab229426)
Interpolated concentrations of native CXCL1 (GRO alpha) in mouse NIH 3T3 cell extract sample based on a 500 μg/mL extract load.
The concentrations of CXCL1 (GRO alpha)were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 89.05 pg/mL in mouse NIH 3T3 cell extract sample
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