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AB216951

Mouse CXCL1 ELISA Kit (GRO alpha)

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(9 Publications)

Mouse CXCL1 ELISA Kit (GRO alpha) is a sandwich ELISA designed to quantify Mouse CXCL1 (GRO alpha) with a sensitivity of 0.66 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 5.47 - 350 pg/mL

View Alternative Names

Gro, Gro1, Mgsa, Scyb1, Cxcl1, Growth-regulated alpha protein, C-X-C motif chemokine 1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51

8 Images
ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
  • ELISA

Supplier Data

ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)

Interpolated concentrations of spiked CXCL1 (GRO alpha) in mouse serum and plasma samples.

The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 386.84 pg/mL in neat serum, 399.45 pg/mL in neat plasma (citrate), 364.34 pg/mL in neat plasma (EDTA), and 505.80 pg/mL in neat plasma (heparin).

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)

Interpolated concentrations of native CXCL1 (GRO alpha) in mouse serum and plasma samples.

The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 48.56 pg/mL in neat serum, 24.34 pg/mL in neat plasma (citrate), 23.67 pg/mL in neat plasma (EDTA), and 176.56 pg/mL in neat plasma (heparin).

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)

Interpolated concentrations of native CXCL1 (GRO alpha) in mouse NIH 3T3 cell culture supernatant sample.

The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : NIH 3T3 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 472.88 pg/mL in neat mouse NIH 3T3 cell culture supernatant.

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)

Example of mouse CXCL1 (GRO alpha) standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
  • sELISA

Lab

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)

Mouse CXCL1 (GRO alpha) standard curve comparison data.

Standard curve comparison between mouse CXCL1 SimpleStep ELISA® kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold sensitivity.

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)

Interpolated concentrations of native CXCL1 (GRO alpha) in mouse NIH 3T3 cell extract sample based on a 500 μg/mL extract load.

The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 89.05 pg/mL in mouse NIH 3T3 cell extract sample.

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL1 ELISA Kit (GRO alpha) (AB216951)

Example of mouse CXCL1 (GRO alpha) standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.66 pg/mL

Range

5.47 - 350 pg/mL

Assay time

1h 45m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CXCL1 ELISA Kit (GRO alpha) ab216951 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse CXCL1 (GRO alpha)in . This SimpleStep sensitivity is 0.66 pg/mL.

How the assay works

Mouse CXCL1 (GRO alpha)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse CXCL1 ELISA Kit (GRO alpha) ab216951 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CXCL1/GRO alpha antibody [EPR16617-85] - BSA and Azide free (Capture) ab243018
- Anti-CXCL1/GRO alpha antibody [EPR16617-115] - BSA and Azide free (Detector) ab243018

CXCL1 is a member of the CXC family of chemokines. Chemokines play roles in normal and pathological processes including allergic responses, angiogenesis, inflammation, tumor growth and metastasis. Mouse CXCL2 and CXCL3 share 67% and 60% sequence homology with mouse CXCL1, respectively. Additionally, Rat CXCL1 is 89% homologous with mouse CXCL1.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" } ]
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Recovery

[ { "sample": "Serum", "range": "88 - 94 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "89 - 98 %", "average": "= 94" }, { "sample": "Cell culture extracts", "range": "95 - 96 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "72 - 90 %", "average": "= 79" }, { "sample": "Citrate plasma", "range": "92 - 97 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "109 - 110 %", "average": "= 110" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab216951-1x96Tests", "assetComponentDetails": [ { "size":"2 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB216951-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB216951-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB216951-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB216951-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB216951-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB216951-CMP07", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB216951-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB216951-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB216951-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXCL1 Capture Antibody", "number":"AB216951-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CXCL1 Lyophilized Recombinant Protein (ab9906)", "number":"AB216951-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXCL1 Detector Antibody", "number":"AB216951-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.
Biological function summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Pathways

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.
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Product protocols

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Target data

Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation (By similarity). Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity.
See full target information Cxcl1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4677 PubMed37542037

2023

Targeting of SLC25A22 boosts the immunotherapeutic response in KRAS-mutant colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Zhou,Yao Peng,Fenfen Ji,Huarong Chen,Wei Kang,Lam-Shing Chan,Hongyan Gou,Yufeng Lin,Pingmei Huang,Danyu Chen,Qinyao Wei,Hao Su,Cong Liang,Xiang Zhang,Jun Yu,Chi Chun Wong

Life (Basel, Switzerland) 13: PubMed37374057

2023

Stimulation of Angiotensin II Type 2 Receptor Modulates Pro-Inflammatory Response in Microglia and Macrophages: Therapeutic Implications for the Treatment of Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulkarim Alshammari,Yohan Han,Timothy W Jones,Bindu Pillai,Duo Zhang,Adviye Ergul,Payaningal R Somanath,Susan C Fagan

Brain sciences 13: PubMed36672133

2023

Resolvin D2 Reduces Chronic Neuropathic Pain and Bone Cancer Pain via Spinal Inhibition of IL-17 Secretion, CXCL1 Release and Astrocyte Activation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Pang,Pengfei Xin,Ying Kong,Zhe Wang,Xiaopeng Wang

Nature communications 13:7648 PubMed36496449

2022

Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Klapproth,Anke Witt,Pauline Klose,Johanna Wiedemann,Nikitha Vavilthota,Stephan R Künzel,Susanne Kämmerer,Mario Günscht,David Sprott,Mathias Lesche,Fabian Rost,Andreas Dahl,Erik Rauch,Lars Kattner,Silvio Weber,Peter Mirtschink,Irakli Kopaliani,Kaomei Guan,Kristina Lorenz,Paul Saftig,Michael Wagner,Ali El-Armouche

Cell death & disease 13:562 PubMed35732633

2022

LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Jia,Sujuan Xu,Ting Ren,Tianyi Pan,Xiaoyan Wang,Yunlu Zhang,Zhouping Zou,Man Guo,Qi Zeng,Bo Shen,Xiaoqiang Ding

Frontiers in pharmacology 12:719842 PubMed34381369

2021

L-Theanine Alleviates IMQ-Induced Psoriasis Like Skin Inflammation by Downregulating the Production of IL-23 and Chemokines.

Applications

Unspecified application

Species

Unspecified reactive species

Yaohan Xu,Jiang Zhu,Jingyi Hu,Ziqi Zou,Yueling Zhao,Lihua Lai,Ping Xu,Yinjing Song,Hao Cheng

Basic research in cardiology 115:67 PubMed33185739

2020

Functional investigation of the coronary artery disease gene SVEP1.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Winkler,Philipp Müller,Amin M Sharifi,Jana Wobst,Hanna Winter,Michal Mokry,Lijiang Ma,Sander W van der Laan,Shichao Pang,Benedikt Miritsch,Julia Hinterdobler,Julia Werner,Barbara Stiller,Ulrich Güldener,Tom R Webb,Folkert W Asselbergs,Johan L M Björkegren,Lars Maegdefessel,Heribert Schunkert,Hendrik B Sager,Thorsten Kessler

Experimental and therapeutic medicine 15:3530-3536 PubMed29545879

2018

Berberine ameliorates neonatal necrotizing enterocolitis by activating the phosphoinositide 3-kinase/protein kinase B signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengzhi Fang,Lili Xie,Chunmei Liu,Chunhua Fu,Wei Ye,Hong Liu,Binghong Zhang

Scientific reports 7:597 PubMed28377603

2017

HMGB1-TLR4-IL23-IL17A axis promotes paraquat-induced acute lung injury by mediating neutrophil infiltration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bailing Yan,Feng Chen,Lijun Xu,Jihong Xing,Xuefu Wang
View all publications
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